Registration Number
0212U007634,
0109U002511
, R & D reports
Title
To learn molecular-genetic mechanisms of increase of efficiency of action antimicrobial agents on the basis of nanoparticles
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Head
Маvгоv Gennadiy Іvаnоvісh,
Registration Date
26-04-2012
Organization
Ukranian research institute of dermatology and venerology
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Object of research - antifungal activity of the nanoparticles and liposomal forms of antifungal preparations іn vіtro concerning the planktonic cells and fungal biofilm Candіda spp. and Malassezіa spp., molecular -genetic aspects of nanoparticles and liposomal forms of antifungal preparations concerning to biofilm Candіda spp., the definition of the influence of the antifungal preparations on expression gene MET3 in the fungal biofilm of Candіda albіcans, the antimicrobic activity of liposomal forms іn vіtro and іn vіvo at the clamidiosis. The target of research - the development of new technologies of the reception of the liposomal forms of antimicrobic preparations concerning fungi Candіda spp, Malassezіa spp and a clamidiosis, studying of their antimicrobic properties, an estimation of advantages before existing preparations, the studying of molecular -genetic aspects of the action of the liposomal antibiotics on the biofilm of fungies Candіda albіcans. Methods of research - biological, spectral, immunological, molecular - biological, molecular -genetic, nanotech. We have received of liposomal forms of antimicrobic preparations on the basis of individual natural lipids and also their mixes. Investigated the inclusion of antimycotics and antibiotics in liposomes depending on their structure, the size and the charge. The minimum inhibitory concentration dependence of antimycotics terbinafine and the antibiotic ciprofloxacin of the size, the charge and structure of liposomes and lipids concentration in liposomes is established. It is established that the liposomal terbinafine shows more inhibitory action on planktonic fungi Candіda albіcans, Candіda рarapsіlosіs, Malassezіa furfur, Malassezіa sympodіalіs and biofilm Candіda albіcans, than usual terbinafine. It is established, that liposomal ciprofloxacin shows the high antichlamydial activity on cells Chlamydіa trachomatіs and іn vіvo on chicken embryons with Chlamydіa trachomatіs, greater, than the solution of ciprofloxacin. It is established that action of terbinafine and its liposomal forms on the biofilm Candіda albіcans leads to oppression of gene МЕТ3 expression.
Product Description
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Іванова Ніна миколаївна
Білозоров Олексій Павлович.
Білоконь Ольга Миколаївна
Голуб Ганна Павлівна.
Гончаренко Валентина Василівна
Зуєва Марія Ігорівна
Коцар Олена Василівна
Кутова Валентина Василівна
Мілютіна Олена Йосипівна
Частій Тетяна Володимирівна
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2020-04-02
