Registration Number
0212U008330,
0112U003465
, R & D reports
Title
The elucidation of molecular interactions in Scots pine (Pinus sylvestris L.) - phytopathogenic bacteria system
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Head
Gout Roman Tarasovych,
Registration Date
19-12-2012
Organization
National Forestry University of Ukraine
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Purpose: to investigate the biological activity of Scots pine antimicrobial peptides (AMP) against bacterial pathogens of conifers, as well as elucidate the features of the expression of antimicrobial peptides under biotic stress and reveal inducers of pine immunity. Methods: physiological and biochemical, molecular genetics, microbiology, genetic engineering. It was found that strains P2 - Bacillus sp.; P10 - Bacillus pumilus and P26 - Paenibacillus sp. can cause disease of one-month and three-old pine seedlings. Stimulative action of bacteria Pseudomonas sp. (P57) on the growth and development of pine seedlings was discovered. This strain of bacteria inhibited the growth of pathogenic fungi Fusarium sp., which causes lodging of pine seedlings, indicating the possibility of using of these bacteria to combat fungal diseases of seedlings. It was shown that stress phytohormones salicylic and jasmonic acid increase the level of pine defensin gene expression defensins. The treatment of pine seedlings with bacteria Pseudomonas sp. causes an increase the level of PsDef1 and PsDef2 (Pinus sylvestris Defensin) gene expression under H. annosum infection. Characteristics of P57strain indicate possible it belongs to a group of bacteria PGPB, which contribute to the development of systemic resistance of plants to pathogens exposure.
Product Description
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Груник Наталія Ігорівна
Гут Роман Тарасович
Ковальова Валентина Андріївна
Шаловило Юлія Ігорівна
Юсипович Юрій Михайлович
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2020-04-02
