Search academic texts

Information × Registration Number 0212U009020, 0110U000988 , R & D reports Title Supramolecular systems in chemistry and biology. Calixarens as the activity modulators of enzyme and transport proteins of smooth muscles. popup.stage_title Head Kosterin Sergiy Oleksijovych, Registration Date 26-12-2012 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 In the experiments carried out with the suspension of the myometrium cell plasmatic membranes we investigated the influence of сalixarene С-107 on the Nа+,K+-АТРase activity. It was shown that this calixarene increased the affinity of the enzyme for the sodium pump conventional inhibitor - ouabain: the magnitudes of the seeming constant of inhibition changed from 26.9 ± 1.3 mM to 10.9 ± 0.6 mM. However the ouabain itself did not influence on the affinity of the Nа+,K+-АТРase for сalixarene С-107. The aim of our investigation was to determine structural features of calix[4]arene C-99 which are important for its inhibition properties relative to Nа+,K+-ATP-ase of uterus myocite plasma membrane. Therefore we studied the effects of calix[4]arenes - С-296, С-297, С-424, С-425, С-426, С-427, which are structural similar to this inhibitor, on mentioned enzyme activity. We have shown that calixarenes С-296 and С-297 which have two additional propoxy groups on the lower rim of macrocycle are less effective inhibitors of Nа+,K+-ATP-ase relative to calixarene C-99. Calixarenes С-425 and С-427 which have on the upper rim of macrocycle three and four phosponic residues respectively also inhibit Nа+,K+-ATP-ase activity less effective as compared to calixarene C-99. Both calixarenes: С-424, which has only two carbonate residues on the upper rim, and С-426, which has on the upper rim ketomethilphosphonate residues instead of hydroxymethilphosphonate residues of calixarene C-99, do not effect on Nа+,K+-ATP-ase activity. We have made respective conclusions concerning the role of certain chemical groups of calixarene C-99 during its interaction with Nа+,K+-ATP-ase. Product Description popup.authors Бабіч Лідія Григорівна Бевза Олександр Васильович Векліч Тетяна Олександрівна Данилович Ганна Вікторівна Данилович Юрій Володимирович Лабинцева Раїса Дмитрівна Шкрабак Олександр Анатолійович Шликов Сергій Георгійович popup.nrat_date 2020-04-02 Close