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Information × Registration Number 0212U009042, 0112U006007 , R & D reports Title Mechanisms of the influence of chitinase-like proteins and redox regulators on the oncogenic properties of the cells popup.stage_title Head Kavsan Vadym Musiyovich, Registration Date 27-12-2012 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The effect of marker proteins CHI3L1 and CHI3L2 on mitogenic properties and proliferative potential of cells have been investigated. Addition of CHI3L1 to the cell culture medium stimulated cell division and, conversely, addition of CHI3L2 inhibited cell growth. 293_CHI3L1 cells which stably produce CHI3L1 protein, are able to anchorage-independent growth in soft agar and to form foci. These features evidenced about oncogenic properties of CHI3L1. 293_CHI3L1 cells generate ROS more rapidly when exposed to menadione than that of the control 293 cells, thus a producing CHI3L1 glycoprotein by these cells leads to modification of their redox properties. Simultaneous addition of both proteins to the cell culture medium inhibits the proliferative effect of CHI3L1 protein. Inhibition of cell growth can be attributed to the activation of apoptosis-associated caspases-3 and -7 in 293 cells which were processed solely by CHI3L2, or in combination with CHI3L1. CHI3L2-activated cells unfastened from agar and subjected to shape change to spherical ones like after application of known apoptotic agent staurosporin. The use of specific inhibitors of apoptotic signal-regulated kinase 1 (ASK1) resulted in inhibition of apoptotic influence of CHI3L2 protein on the cells. It can be assumed about ASK1-mediated influence of CHI3L2 protein on the apoptosis development in activated cells. It has been revealed that CHI3L2 initiates RI3K signaling cascade by activating of AKT phosphorylation in 293 cells and U373 cells. Comparison of kinetics for dose- and time-dependent influence of chitinase-like proteins CHI3L1 and CHI3L2 on AKT phosphorylation showed the differences in AKT activation profiles which probably reflect the different impact of CHI3L1 and CHI3L2 upon AKT function.5481 Product Description popup.authors Єршов Антон Вікторович Арешков Павло Олександрович Балинська Олена Вікторівна Бойко Оксана Іванівна Букреєва Тетяна Василівна Дмитренко Володимир Володимирович Чаусовський Тімур Йосипович popup.nrat_date 2020-04-02 Close