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Information × Registration Number 0212U009095, 0112U002802 , R & D reports Title Identification of genetic sequences that determine the quality characteristics of grain and resistance to stress factors in maize popup.stage_title Head Morgun Bogdan Volodymyrovych, Registration Date 29-12-2012 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 Samples of 200 experimental maize forms presented by the Maize Company have been screened for the presence of genetic sequences that determine the quality characteristics of grain (wx and fl-2 genes) and resistance to stress factors: herbicide tolerance (bar (pat), epsps genes) and insect resistance (cry genes). The total DNA was purified from maize living plant tissue. Primers to detect genes wx, fl-2, bar (pat), epsps, mepsps, CP4 epsps, cryIA(b), cry1F, cry1A.105, mcry3A, cry2Ab2, cry3Bb1, cry34Ab1, cry35Ab1 were designed and selected. Multiplex and Touchdown PCR for simultaneous determination of few genes were worked out. PCR amplification of sequences was carried out to determine the presence or absence of transgenes (bar, epsps, cry) and wx and fl-2 genes. Transgenes and wx, fl-2 genes detection protocol was composed for experimental maize forms. The description of experimental maize forms for the presence of transgenes and wx, fl-2 genes was prepared. Product Description popup.authors Банникова Марія Олександрівна Марковський Олексій Вікторович Нітовська Ірина Олександрівна Степаненко Антон Ігорович Федоренко Тетяна Валеріївна Череп Микола Никифорович popup.nrat_date 2020-04-02 Close