Registration Number
0213U000950,
0108U001669
, R & D reports
Title
Development of biotechnology of cloning in vitro and cultivations of power and landscape gardening cultures with application in alternative energetic and municipal gardening.
popup.stage_title
Head
Melnychuk Maksym, Доктор біологічних наук
Registration Date
23-04-2013
Organization
Research Institute of Plant Science, Soil and Sustainable Nature Management Education and Research Institute of Plant Science, Environment and Biotechnology National university of life and environmental sciences of Ukraine.
popup.description2
The object of research - technology of in vitro cloning and adapting of economic valuable crops produced on virus-based somaclonal variation in vitro, differentiation and identification of plant genotypes and phytoviruses. The purpose - establishing the main fast-growing species of trees and shrubs with a relatively short period of growth and landscape crops with valuable features, the development of conditions of obtaining in vitro plant material for furthe r breeding, DNA analysis of SSR-markers of cultivated and wild forms to differentiation and identification the genotypes. Research methods - methods of cells culturing, tissues and organs of plants in vitro, agriculture technology methods of growing plants and processing of raw materials, histochemical methods for detecting nucleic acids, polysaccharides, lipids and proteins, methods for determination of the mitotic index and anaphase study method of chromosome aberration, extraction of nucleic acids (DNA and RNA), the amplification of DNA fragments by using PCR, reverse transcription, electrophoretic analysis of the amplification products (in agarose and polyacrylamide gel electrophoresis), cluster and phylogenetic analyzes, processing of the experimental data with using of the modern computer program s and web-based resources. As a result the theoretical principle was developed the methodological technology of cloning and adapting of decorative plants and fast-growing tree species for greening and alternative energy were modified. Methodical approaches of the analysis of changes in willow genotype (Salix viminalis L.), breeding in culture using aseptic techniques to analyze genetic polymorphism of DNA by PCR (RAPD-, ISSR-PCR, AFLP and SSRP) were developed. The methodology of hops genetic certification (Humulus lupulus L.) of Ukrainian selection using microsatellite analysis and variants of molecular genetic passports was developed. The diagnostic test system, based on polymerase chain reaction (PCR), was developed for detection of apple mosaic virus (AMV) andPrunus necrotic ring-spot virus (PNRSV). Scope - Agriculture i forestry.
Product Description
popup.authors
Антіпов Ігор Олександрович
Білоус Світлана Юріївна
Вітковський Олександр Володимирович
Дрозд Петро Юрійович
Дубін Олексій Вікторович
Клюваденко Андрій Андрійович
Ліханов Артур Федорович
Оверченко Віталій Віталійович
Оверченко Оксана Василівна
Рожко Юрій Володимирович
Чорнобров Оксана Юріївна
Шульга Валентин Миколайович
popup.nrat_date
2020-04-02
