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Information × Registration Number 0213U001024, 0110U000232 , R & D reports Title Molecular genetic mechanisms of radiation-associated oncohematological pathology forming in victims of the Chernobyl NPP accident. popup.stage_title Head Klymenko Sergiy Viktorovich, Доктор медичних наук Registration Date 09-01-2013 Organization State institution "National research centеr for radiation medicine of the National academy of medical sciences of Ukraine " popup.description2 Object: сlinical, hematological, molecular-genetics, cells culture indicators for persons exposed to ionizing radiation due to the Chernobyl accident and patients without radiation history with myelodisplastic syndrome, polycythemia vera, essential thrombocythemia and idiopathic myelofibrosis. Purpose: to determine the molecula- genetics characteristics of oncohematological pathology of myeloid origin forming in persons exposed to ionizing radiation. Methods: clinical, hematological, molecular-genetics, cells culture, immunologic, and statistics. We have used immunoferment analyzer Humareader (Germany), thermocycler Gene-Amp PCR 2700 (Singapore), microscope "Sedival" and "Standard 25" Carl Zeiss, Jena (Germany). The JAK2 V617F mutation was detected by multiplexed allele-specific polymerase chain reaction. The method of detection JAK2 V617F mutation was validated as a marker of spontaneous and radiation associated pathology, in particular polycythemia vera (specificity - 99,14% and 94,73%, sensitivity - 95,65% and 96,29% accordingly), idiopathic myelofibrosis (specificity - 100% for both groups, sensitivity - 61,9% and 33,3% accordingly), essential thrombocythemia (specificity - 100% for both groups, sensitivity - 65,62% and 50% accordingly). The cultivation of hematopoietic stem cell of peripheral blood in an environment with autoserum (clusters ?15,37±2,95) has sensitivity - 100% and specificity - 83% for cases with under-representation of a major diagnostic criteria, that can be used for diagnostics of Ph-negative chronic myeloproliferative disorders. Product Description popup.authors Балан В. В. Дмитренко І.В. Костюкевич О.М. Любарець Т.Ф. Міщенюк О.Ю. Ніколаєнко-Камишова Т.М. Прокопенко І.М. Федоренко В.Г. Шолойко В.В. popup.nrat_date 2020-04-02 Close