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Information × Registration Number 0213U003137, 0112U002963 , R & D reports Title Portative analyzer based on amperometric enzymatic biosensors for the quality control of beverages in wine-making. Isolution of preparative guantities of the stabilized enzymes. popup.stage_title Head Sibirny Andrei A., Registration Date 24-01-2013 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Objects of the investigation: enzymes of bioanalytical importance - alcohol oxidase (AO), glycerol oxidase (GO), recombinant flavocytochrome b2 (FC b2), recombinant glycerol dehydrogenase (GDH). The aim of the work : to create the compact analyzer on the base of amperometric biosensors for quality control of drinks in wine industry. The aim of the work (part of ICB NASU): to for obtain the preparative quantities of stabilized enzymes. The aim of the stage for 2012 year: to carry out preparative isolation of the required enzymes to create the bioelements of sensors. To compare the results of real wine's samples analyses made by multi-biosensor and other approaches (the developed enzymatic method and reference. Methods of investigations: for microbiological, biochemical, physical-chemical, analytical, enzymatic. As a result of the work, the growth conditions for recombinant strain of S. cerevisiae W303GO, GDН-producer, were optimized; the cells of microbial strains containing the elevated level of bioanalytically important enzymes were cultivated in preparative quantities; large-scale isolation and purification of some enzymes were carried out, namely: GO, FC b2, recombinant and different forms of AO; the collaborator partner (Laboratory of Bio-molecular Electronics, Department of Translation Mechanisms of Genetic Information IMBG NASU) was provided with highly-purified AO, GO and FC b2 preparations - as bio-elements for multi-biosensor fabrication and testing; comparative study of glycerol content by different methods was made. Product Description popup.authors Іваш М.Ф. Гайда Г.З. Гончар. М.В. Демків О.М. Закальська О.М. Карковська М.І. Стасюк Н.Є. popup.nrat_date 2020-04-02 Close