Search academic texts

Information × Registration Number 0213U004491, 0113U006147 , R & D reports Title Molecular mechanisms of realization genetic information popup.stage_title Head El'skaya Anna Velentynivna, Доктор біологічних наук Registration Date 23-12-2013 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Gene expression is a basic process in the cells of the organism. Organism's life depends on this process, the regulation of which occurs at all levels, namely at the level of transcription of mRNA splicing, translation ( initiation, elongation, termination), the stability of proteins and their after-translational modifications. The main goal of the research was to determine the molecular mechanisms of realization of genetic information at the level of transcription regulation process investigation (including genetic and epigenetic changes in DNA), transmission and signaling status of the cell in health and disease. It was necessary to clarify a number of issues on this way, including: - Molecular mechanisms of RNA- dependent post- transfer correction by leucyl -tRNA synthetases prokaryotes and archaea and mechanisms of pre- transfer correction by leucyl -tRNA synthetases . - The role and molecular mechanisms of action of different isoforms of ribosomal protein kinase S6 - S6K1, S6K1d15, S6K2 on the state of RI3K dependent signaling cascade in the regulation of mRNA translation. - Explanation of the spatial structure of the complex translation elongation factor 1A2 with HDF using crystallographic analysis. - Elucidation of the molecular mechanisms of complex formation of small GTPase RhoU adaptive intersectin protein 1 (ITSN1), associated with Alzheimer's disease and Down syndrome, which determines the effect of the interactions of RhoU ITSN1 on their subcellular localization and constitutive endocytosis. Determination of the sites of calcium -dependent phosphorylation of ITSN1 and investigation of the impact of ITSN1 gene expression on the formation and growth of dendrites and axons, and formation of dendritic trees for neuronal cell cultures. - Identification of potential markers of aggressiveness and metastasis of human epitelial tumors (carcinoma of the kidney, prostate, colon tumors ) with the expression profile of genes which are involved in major onco -associated signaling pathways and the nature of the genetic and epigenetic disturbances in tumors of epithelial origin by NotI - microchips method. - The role of oncogene overexpression of CHI3L1 gen, and CHI3L2 gen homologous to it, in the malignant transformation of brain cells and the role of the interactions of hitynaza-like proteins with signaling cascades PI3K/AKT and RAS / MAPK in the acquisition of proliferative and invasive properties by tumor cells. Product Description popup.authors А.В.Риндия Б.С.Негруцький В.І.Кашуба В.В.Філоненко В.М.Кавсан Г.В.Єльська Г.Д.Яремчук М.А.Тукало popup.nrat_date 2020-04-02 Close