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Information × Registration Number 0213U005343, 0111U000807 , R & D reports Title Develop a system for monitoring and control of bacterial contamination of feed and feed additives popup.stage_title Head Kutzan Olexandr, Доктор ветеринарних наук Registration Date 10-04-2013 Organization Nationali Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" popup.description2 Object of study - salmonellosis animal genome of Salmonella species Salmonella enterica serotypes Salmonella Dublin, Salmonella Heidelberg, Salmonella Infantis, Salmonella Virchow, Salmonella Typhisuis, Salmonella Gallinarum-pullorum, Salmonella Typhi.Purpose - to work out the method of indicating bacteria of the genus Salmonella and identification of Salmonella species Salmonella enterica serotypes Salmonell Enteritidis and Salmonella Typhimurim by polymerase chain reaction for monitoring the quality and safety of animal products, feed and feed ingredients of animal origin.Monitored the study of feed and their constituents about the availability of salmonella contamination serovariantiv Salmonella Enteritidis and Salmonella Typhimurium. Total examined 185 samples (prepared animal feed, meat and bone meal, fish meal, samples zernosumishey, meal).The presence in the samples of food pathogens bacterial infections of the genera Salmonella, Escherichia, Proteus, Enterobacter, Citrobacter, Staphylococcus, but the proportion of them were different. On average, 21% of the investigated samples isolated Salmonella from 68% - other members of the family Enterobacteriaceae, 8% - Corynebacterium, a much smaller number - acinetobacter and staphylococci.Studies on artificial contamination of samples of food (ready-mixed and zernosumishey) Salmonella cultures and comparative identification of Salmonella in their standard bacteriological methods and PCR. According to the research found that the artificial contamination of samples mixed sensitivity bacteriological method and PCR was determined at the same level, in concentrations ranging from 2h105 CFU / g to 2h109 CFU / g for S. Typhimurium and from 2h106 CFU / g to 2h109 CFU / g for S. Enteritidis.Estimated primers for species-specific detection of bacteria of the genus Salmonella serovariantiv Salmonella Dublin, Salmonella Heidelberg, Salmonella Infantis, Salmonella Virchow, Salmonella Typhisuis, Salmonella Gallinarum-pullorum, Salmonella Typhi. Product Description popup.authors Ареф'єв В.Л. Герілович А.П. Глєбова К.В. Заремба І.А. Майборода О.В. Петренчук Е.П. popup.nrat_date 2020-04-02 Close