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Information × Registration Number 0213U006775, 0110U006119 , R & D reports Title Creating a prototype of test systems for DNA analysis of genome rearrangements that cause monogenic hereditary diseases and hereditary predisposition to the development of the most socially important human diseases popup.stage_title Head Livshits Ludmila Avramovna, Registration Date 30-12-2013 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The goal of the project is to develop a prototype test system for the analysis of allelic variants of genes MTHFR, F5, F2, eNOS, ACE, ESR, ITGA2 and CYP2C9 (members of the panel of markers of genetic susceptibility to stroke) and to create panels of DNA samples of individuals with different allelic variants of genes MTHFR, F5, F2, ESR, and ITGA2 CYP2C9. The diagnostic methods comprising different variants of polymerase chain reaction (PCR) analysis, restriction fragment length polymorphism (RFLP) as well as modifications in the methods of real-time PCR in the embodiment of TaqMan were developed for analysis of allelic variants of MTHFR, F5, F2, eNOS, ACE , ESR, ITGA2 and CYP2C9 - stroke susceptibility candidate-genes. The synthesis and development of the design of PCR primers for exon sequences of different genes MTHFR, F5, F2, eNOS, ACE , ESR, ITGB3 and CYP2C9 and fluorescently - labeled probe for real time PCR of gene F5 and F2 in the embodiment of TaqMan were carried out. Developed methods were tested on reference DNA samples with the relevant mutations, providing independent analysis of alternative methods. The panel of DNA samples of individuals with known mutations in genes MTHFR, F5, F2, ACE, eNOS, ESR1, ITGA2 and CYP2C9 was established. On the basis of the developed techniques developed prototypes of test systems for molecular diagnosis of the genetic susceptibility to stroke that do not require expensive equipment and can be implemented in specialized medical institutions. Keywords: prototype test system, markers of genetic susceptibility to stroke, DNA diagnostics, PCR, PCR-RFLP analysis, real-time PCR, TaqMan. Product Description popup.authors Грищенко Наталія Володимирівна Кравченко Сергій Афанасійович Кучеренко А.М. Нечипоренко М.В. Павло Феліксович Пампуха Володимир Миколайович Соловйов Олександр Олександрович Чернушин С.Ю. popup.nrat_date 2020-04-02 Close