Registration Number
0214U000101,
0113U001998
, R & D reports
Title
Collaboration research in the molecular epidemiology and diagnostic technique for PRRSV and BVDV disease
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Head
Gerilovych Anton Pavlovych,
Registration Date
16-01-2014
Organization
Nationali Scientific Center "Institute of Experimental and Clinical Veterinary Medicine"
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Object of research - epizootic of Bovine virus diarrhea . Goal - to study , isolation and genetic characteristic of virus strains of Bovine virus diarrhea, that circulating in both countries (China and Ukraine) and their phylogenetic comparison, the development and introduction of new methods for detecting viruses biological materials. Research methods - serology, molecular - genetic, phylogenetic. The 224 samples of clinical material from cattle of different age groups from 11 farms areas of Ukraine were studied. The 184 serum samples from animals of three farms Kharkiv region were studied for detection antibodies to the pathogen of bovine diarrhea with ELISA. Eighteen PCR positive samples further were investigated by sequencing. The phylogenetic analysis of the sequences was performed. The phylogenetic trees based on the sequences of the Ukrainian and Chinese isolates were constructed. The PCR test system for detection of Bovine virus diarrhea was developed. The PCR test system for detection of Bovine virus diarrhea was developed.
Product Description
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Болотін Віталій Ігоревич
Бузун Андрій Ігоревіч
Горайчук Ірина Василівна
Кольчик Олена Володимірівна
Кучерявенко Вікторія Вікторовна
Кучерявенко Роман Олексійович
Солодянкін Олексій Сергійович
Стегній Марина Юріївна
Шутченко Павло Олександрович
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2020-04-02
