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Information × Registration Number 0214U002307, 0113U002317 , R & D reports Title Definition of the role of genetic and epigenetic changes in the genome of somatic cells in the pathogenesis of chronic lymphoproliferative diseases, their diagnostic and prognostic significance popup.stage_title Head Bebeshko Volodymyr; Minchtnko Zhanna, Registration Date 28-01-2014 Organization State institution "National research centеr for radiation medicine of the National academy of medical sciences of Ukraine " popup.description2 Object of research - the biological material of 90 patients with multiple myeloma, chronic lymphocytic leukemia, non-Hodgkin's malignant lymphoma. The purpose of work - on the basis of studies of molecular-cytogenetic changes in the genome of the substrate cells, identify markers associated with the development of chronic lymphoproliferative pathology, and to evaluate the diagnostic value. Methods of research - molecular-cytogenetic, cytogenetical, immunological, immunological, hematological, cultural and statistical. The results of the research. On the basis of the data of clinical and laboratory examinations are determined priority groups surveillance among patients with CLL, MM, NZL. According to the results of cytogenetic and molecular cytogenetic studies of the monitoring group established limits false positive signals ranging from 0.4% to 3.3%. For translocation of samples is defined exclusively pattern of signals 1Oх1Gх1F with fluctuations in the limits from 16.10% to 19,30%. In metaphase plates such distribution of signals is not registered, confirmed that its a false-positive. In the genome of somatic cells of patients CLPN revealed the presence of both quantitative and structural chromosomal abnormalities. Identified deletion of the gene ТР53 45 % persons. Of them clonal changes of TR 53 defined in 28,33% of patients with CLPN: 45 % of patients with B-CLL, 35% - B-NHL, and 5% - MM, that considerably exceeded the values of the error of the method (0,08 %, r<0,01). Clonal anomalies of gene ТР53 significantly more often registered among patients B-CLL compared with a group of patients MM (45,00 % and 5.0%, respectively), r<0,05. Chromosomal abnormalities are detected in 80% of patients MM, mainly quantitative changes of chromosomes 3, 8, 11 and 13. 32.5% of patients MM defined IgH translocation involving chromosomes 4, 11 and 16. Most often observed translocation t(4;14). In most patients, the presence of translocations was associated with hyperdiploid type of chromosomal aberrations. Defined reliable correlation between abnormalities of chromosome 13 (del13q34) and translocation t(4;14), as well as between the translocation t(14;16) and anomalies (monosomy,trisomy) chromosome 16, at p?0,05. Data of immunogenetic analysis indicates normal distribution of phenotypes system ABO in persons with B-CLL Hodgkins and the likely increase in carriage phenotype O(I) in patients MM compared with the control group. Established one hundred percent prevalence of allele HLA-A * 02. Were proposed original modification of the method of hybridization, which significantly improved the quality of drugs and reduce the risk of erroneous detection of false-positive or negative results. Product Description popup.authors Балан Валентина Володимирівна Дмитренко Олена Олександрівна Мішаріна Жанна Анатоліївна Сітько Валентина Віталіївна Шляхтиченко Тетяна Юріївна popup.nrat_date 2020-04-02 Close