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Information × Registration Number 0214U003617, 0113U002555 , R & D reports Title The development and trial exploitation of a portative analyzer based on amperometric enzyme biosensors for quality control of beverages in winery. Development of biosensor methods of arginine analysis in wine by using arginine-hydrolyzing recombinant enzymes. popup.stage_title Head Sibirny Andrey, Registration Date 07-02-2014 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Object of study: recombinant enzymes of bioanalytical value - flavocytohrome b2 (FC b2), glicerol dehydrogenase (GDH), arginase I (arginase), argininedeiminase (ADI). Purpose of the research: development of biosensor methods of arginine analysis in wine by using arginine-hydrolyzing recombinant enzymes. As a result of this work: The cells of recombinant yeast strains - over-producers with a high content of target enzymes were obtained; FC b2 and arginase were isolated from the over-producers yeast strains H. polymorpha "tr1" and NCYC 495 pGAP1-HsARG1(leu2car1 Sc:LEU2), respectively; АDІ Mycoplasma hоminis - from the cels of E. coli BL-21; GDH - H. рolymorpha - from the W303 (HpGDH) Saccharomyces cerevisiae were purified; Preparations of GDH, ADI and FC b2 were transferred to the laboratory of Biomolecular Electronics Department of the Translation Mechanisms of Genetic Information NAS of Ukraine to carry out the research work; The methods of arginase, ADI and mediators immobilization on the surface of amperometric transducer were optimized. The new enzymatic methods for L-Arg assay based on arginase have been developed. Proposed enzymatic methods were applied to the samples of wines for L-Arg testing. According to the results of research 3 articles and 4 abstracts were published. Product Description popup.authors Іваш М.Ф. Борецький Ю.Р. Гайда Г.З. Гончар. М.В. Карковська М.І. Свінтозельська М.М. Стасюк Н.Є. Фаюра Л.Р. popup.nrat_date 2020-04-02 Close