Search academic texts

Information × Registration Number 0214U005436, 0110U005553 , R & D reports Title Complex project: Interaction between nanoscale materials with normal and tumor cells, development of methods of their delivery safety of their use. popup.stage_title Head Chekhun Vasyl Fedorovych, Registration Date 29-12-2014 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology popup.description2 We studied features of interaction between nanomaterials of different origin (fullerenes, nanotubes, gold nanoparticles, magnetic fluid) with normal and malignant cells. We showed that studied nanomaterials had different impact on cells, which in part dependent on the origin of the factor, but mainly was associated with its concentration and cell histogenesis. We found that all nanomaterials in low concentrations stimulated proliferative effects in cells of mesenchimal origin, while high concentrations of nanomaterials suppressed proliferation of cells of epithelial origin. We proposed methodology for evaluation of possible toxicity of nanomaterials, which based on studies of their impact on metabolic, phenotypic and cytogenetic characteristics of normal and tumor cells. Using dark-field microscopy we showed that gold nanoprisms were able to accumulate on the surface of malignant human lymphoid cells and did not interact with normal lymphocytes from healthy donors. Such tropism of nanogold to malignant cells might allow us to perform selective transport of antitumor drugs to tumors by their sorption on nanoparticles. We demonstrated that acceptation of functionalized magnetite nanoparticles by human serum albumin (HSA) took place in the second domain of the protein globule. At the same time their interaction with albumin and development of complexes between HAS and gold, carbon nanotubes and fullerenes was accompaie by severe changes of the native architectonics of protein globule. We showed that nanospheres synthesized with HSA with different degree of cross-linkage demonstrate higher acceptor activity towards cell membranes of malignant cells and higher transport capacity towards hydrophobic and hydrophilic substances compared to native albumin. After incorporation of magnetite nanoparticles into nanosferes, they saved their thermo, electro and magnetic properties, what revealed their perspectives in hyperthermia and vector therapy of patients with malignant neoplasms. During interaction of normal and tumor cells with carbon, iron and gold nanoparticles we noticed some biochemical differences, especially in normal cells. Multiwall carbon nanotubes and magnetic fluid caused increase of peritoneal macrophages numbers, free radicals generation by macrophages in liver and blood. The last mentioned was accompanied by imbalance of antioxidant ferments (superoxide dismutase and catalase) functioning. Gold nanoparticles caused impact predominantely on Echrlich ascetic carcinoma cells and resulted in biochemical changes in tissues of studied animals during tumor growth. We found increase of Echrlich ascetic carcinoma cell numbers, increase of free radicals generation and activation of XOR ferment, which predominantely conststed of oxidase isoform, which took part in generation of reactive oxygen and nitrogen species. Multiwall carbon nanotubes reduced numbers of Echrlich ascetic carcinoma cells. Applcation of all studied nanoparticles resulted in redistribution of XOR isoforms activity towards dehydrogenase form, what could be associated with reduction of free redical generation. Acquired data of the fundamental study should be taken into account during further studies, aimed at development of vector nanosystems of antitumor drugs delivery to biological targets, based on nanomaterials. Product Description popup.authors Єлейко-Хемич Любомира Олегівна Бездєнєжних Наталія Олександрівна Гамалея Микола Федорович Ганжа Олена Борисівна Главін Олексій Анатолійович Демаш Дмитро Валерійович Дружина Микола Олександрович Ковальова Оксана Анатолівна Кудрявець Юрій Йосипович Лісовенко Галина Степанівна Лихова Олександра Олександрівна Лук'янова Наталія Юріївна Маковецька Людмила Іванівна Мельник Наталія Миколаївна Михайленко Віктор Михайлович Налєскіна Леся Анатоліївна Прокопенко Ігор Вікторович Сарнацька Вероніка В'ячеславівна Семесюк Надія Іванівна Танасієнко Ольга Анатоліївна Тодор Ігор Миколайович Чехун Василь Федорович Швець Юлія Вікторівна Шишко Євгенія Денисівна Штонь Ірина Олександрівна Юшко Лариса Олександрівна popup.nrat_date 2020-04-02 Close