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Information × Registration Number 0214U005904, 0110U006119 , R & D reports Title Creating a prototype of test systems for DNA analysis of genome rearrangements that cause monogenic hereditary diseases and hereditary predisposition to the development of the most socially important human diseases popup.stage_title Head Livshits Ludmila Avramovna, Registration Date 26-12-2014 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The goal of the project is to develop diagnostic methods for analysis of polymorphic variants of the MTHFR, F5, F2 genes that belong to the markers of genetic predisposition to cardiovascular diseases by multiplex ligase zond-dependable ampliphication. For this analysis to be conducted the design of specific LPO і RPO oligonucleotide zonds and promers was developed considering the temperature of melting of the LHS та RHS sequences, analysis of their secondary structure and probability of the tautomerization between guanine and timidine residues. The composition and the concentration of the key components for the ligase and polimerase chain reaction were optimized - LPO та RPO zonds and primers, thermostable DNA-ligase and thermostable DNA-polimerase. Temperature, time regimes for the ligase and polimerase chain reaction were optimized. The developed methods were verified with the DNA samples of different allel variants of the investigated polymorphisms using referred samples independently analyzed by alternative methods. Those methods can be used for development of test systems of DNA markers for genetic predisposition to cardiovascular diseases. Product Description popup.authors Грищенко Наталія Володимирівна Кравченко Сергій Афанасійович Кучеренко Анастасія Михайлівна Лівшиць Ганна Борисівна Нечипоренко Марина Пампуха Володимир Миколайович Чернушин Сергій Юрійович popup.nrat_date 2020-04-02 Close