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Registration Number

0214U005992, 0110U006090 , R & D reports

Title

Research of interaction features of nanosize particles with the cultivated cells

Head

Goltsev Anatoly M.,

Registration Date

16-01-2015

Organization

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine

Description

The research objects are cryopreserved fibroblast cells, rats and gold nanoparticles (Au NPs). The research aim is a study of possibility to use of cryopreserved fibroblast culture with Au NPs for therapy of experimental burns in rats. The research methods are cultural, histological, biochemical methods, light and fluorescent microscopy, flow cytometry. The results of the research work. The effect of cryopreserved fibroblast cultures with NPs Au on regenerative processes at their application on the burn surface in animals was researched. Application of cryopreserved cells fibroblasts with Au NPs (6 µg / ml) on burn wounds in rats resulted in decreasing of the burn area to the 21st day (in 2.1 times) compared with the control group (no treatment). Microscopic study of skin sections after application of cryopreserved cells fibroblasts with Au NPs indicated the complete epithelization of burns. Wound defect was completely replaced by young connective tissue in which fiber bundles of collagen type I were organized and had intensive staining. In groups of animals with treatment, leucocyte number and total protein level were normalized, indicating the completion of inflammation in wound. Investigation of application of cryopreserved fibroblast and cryopreserved fibroblast with Au NPs on the surface of the burn showed that in both cases the investigated preparates stimulate the wound healing. The use of cryopreserved fibroblasts had a less pronounced effect compared with cryopreserved fibroblasts with Au NPs, which was reflected in a slower regeneration of burn surface and moderate lymphocytic infiltration of granulation tissue, while on the site of the burn of the animals after application of cryopreserved fibroblast culture with Au NPs was observed almost complete epithelization of the wound.

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Волкова Н. О.
Павлович О. В.
Ревенко О.Б
Юхта М.С.