Registration Number
0214U006182,
0108U008526
, R & D reports
Title
Features of repair enzyme O6-alkylguanine-DNA-alkyltransferase expression in conditionally normal and tumor cells
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Head
Lukash Lyubov Leonidovna, Доктор біологічних наук
Registration Date
17-02-2014
Organization
Institute of Molecular Biology and Genetics of NASU
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It was investigated the expression level changes of O6-methylguanine DNA methyltransferase (MGMT) trepair enzyme under the influence of carbohydrate-binding proteins (growth factors, cytokines, lectines) and in process of cell line stabilization in vitro. It was shown the correlation of mutagenesis level changes and this repair enzyme expression level on the different stages of murine and human cell line stabilization. This fact supports the hypothesis that MGMT took place in mutagenesis processes during cell culture adaptation to in vitro conditions. In tumor and normal tissues, and in some standart tumor cell lines in vitro the presence of unknown protein recognizing by monoclonal anti-MGMT antibodies was shown. Because this unknown protein was shown to be induced by alkylating agents and another biologically active compounds, it was supposed to be involved in bulky alkylated DNA adducts.5481
Product Description
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Карпова Ірина Сергіївна
Кочубей Тетяна Олександрівна
Лило Валентина Володимирівна
Мацевич Лариса Леонідівна
Півень Оксана Олександрівна
Підпала Ольга Володимирівна
Рубан Тетяна Афанасіївна
Сухорада Олена Михайлівна
Яцишина Анна Петрівна
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2020-04-02
