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Information × Registration Number 0214U006184, 0109U008621 , R & D reports Title The role of the biological active substances in the regulation of the physiological functions in the norm and at the different pathological stations popup.stage_title Head Sobolev Valeriy Ivanovich, Registration Date 17-02-2014 Organization Donetsk National University popup.description2 The purpose of the work is the studying of features of interaction between various biologically active agents in regulation of a functional state of neuromuscular and pituitary-thyroid systems, and also the psychoemotional status of the animals. The research problems are the research of modulating influence of thyroxine on the manifestation of the effects of a chronic hypercorticoidism on the electrophysiological parameters of a skeletal muscle, the studying of nature of relationship in hormonal couples of the system "a thyrotropin - thyroid hormones" at the human, the research of individual sensitivity of the white rats to the action of stressful factors of various nature. Methods of the researches: electromyography in the conditions of in situ, the immunofermental analysis, ethological methods. Are developed and created: - the method of the reproduction of hypercorticoidism and moderated hyperthyroid state at the animals; - electromyography method in the conditions of in situ; - the method of the reproduction of stressful states at animals; - ethological methods of the research of a psychoemotional condition of animals; - the experimental researches have been conducted and the experimental material has been statistically treated and analyzed by the study of electrophysiological parameters of the skeletal muscle at an experimental hypercorticoidism and in the conditions of complex application of a synthetic glucocorticoid of dexamethasone with the thyroxine; it has been carried out the analysis of efficiency of thyroxine for smoothing of negative effects of dexamethasone on a skeletal muscle; - the experimental researches have been conducted and the experimental material has been statistically treated and analyzed concerning the nature of relationship in hormonal couples of system "a thyrotropin - thyroid hormones " at the human; - it has been studying the behavioral reactions of the animals in the conditions of various stressful states taking into account their initial emotional status. The most essential results of the researches. It was established that the experimental hypercorticoidism was accompanied by lengthening of the latent period, decrease in amplitude and shortening of the duration of M-response of the muscle, deterioration of reliability of a neuromuscular transmission, and also essential increase in a frequency of the polyphase potentials (to 82% after 2 months of injection of dexamethasone), testifying in favor of possible delay of a neuromuscular transmission, decrease in excitability of muscular fibers and their partial switching off from the general excitement of a muscle. Application of thyroxine in a complex with dexamethasone prevented the lengthening of the latent period, the decrease of amplitude and the shortening of the duration of the M-responses, taking place at the isolated injection of dexamethasone and also caused an essential decrease of frequency of polyphase potentials of the muscle. Besides, thyroxine prevented the development of the essential decrement of the amplitude and the lengthening of the latent period of M-responses in the process of indirect electric stimulation of the muscle with a frequency of 4 imp/s, typical at the isolated application of dexamethasone. Within physiological norm of fluctuations of the level of a thyrotropin of blood of young men (n=98) regulation in hormonal TTG-T3 couple is carried out on the basis of the principle of positive feedback from correlation coefficients higher than 0,90. At achievement of the TTG level of extreme limit of norm (higher than 4,0 mcIU/ml) the regulation type in hormonal TTG-T3 couple changes on opposite and is carried out on the basis of the classical principle "the plus-minus interaction". Regulation of the level of the free triiodthyronine in hormonal couple "TTG-T3free" it is most precisely carried out within physiological concentration of circulating TTG; on limits of physiological norm of fluctuations of a thyrotropin support of constancy of the level of triiodthyronine takes place with the smallest accuracy. The last can be interpreted from the point of a view of approach at extreme physiological (still normal) values of the TTG level of a phase of unstable regulation that is a sign of approach of a critical point in regulatory system. Unlike a free form of triiodthyronine the level of the connected form of a hormone in parameters of variability is least precisely supported only at the end of a scale of physiological concentration of TTG, i.e. in the field of higher concentration. On the limits of physiological norm of the TTG level the relation of an active form of triiodthyronine to common form (T3free/T4common) is supported with high degree of a variation whereas in the middle of a scale of concentration, process of regulation is carried out more precisely. The dependence between free thyroxine and the TTG level of a blood has difficult character and in different sites of a scale of concentration of TTG is described by the equations of a straight line having an opposite orientation. At the end of a scale of physiological fluctuations of the TTG level of blood the concentration of the common thyroxine is maintained less steadily, with high parameters of statistical variability, than in the middle of a scale of concentration of TTG. The physiological efficiency of the action of the unit of TTG on a thyroid hormone regulated in different sites of a scale of concentration significantly differs. In particular, at the beginning of a scale of concentration of TTG its effective force exceeds that at the end of a scale on many times. In the relations between a thyrotropin of a hypophysis and, finally, regulated it free triiodthyronine are available important features: the price of the regulatory mechanism is high as maintenance of an available circulator form of free triiodthyronine requires the presence of higher concentration of TTG, than at cases with thyroxine and the common triiodthyronine. The reactions of rats to a stress were various depending on initial degree of expressiveness - high, average or low - behavioural indexes in used tests: in the raised crosswise labyrinth, the made a hole field and in Porsolt's test. It is established that as a result of action of an emotional stress of various genesis the depressive state develops mainly at initially highly active animals in the conditions of the made a hole field. The social stress induces a depression at groups of experimental animals with initially low and average depressive levels in Porsolt's test. Use of rats with initially various levels of expressiveness of psychoemotional characteristics are new model of a psychical depression. DEXAMETHASONE, THYROXINE, TRIIODTHYRONINE, THYROTROPIN, HYPERTHYROID, HYPERCORTICOIDISM, SKELETAL MUSCLE, STRESS, DEPRESSION, BEHAVIORAL REACTIONS. Product Description popup.authors Дерев'янченко Ірина Анатоліївна Капланец Ірина Вікторівна Труш Віктор Іванович Труш Віра Володимирівна Фролова Галина Олександрівна popup.nrat_date 2020-04-02 Close