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Information × Registration Number 0214U008367, 0110U006325 , R & D reports Title Studying the selective autophagic degradation of organelles and cytosolic proteins in yeasts Pichia pastoris and Saccharomyces cerevisiae popup.stage_title Head Sibirny Andriy Andriyovych, Registration Date 22-12-2014 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Though most of the autophagic processes are studies at the model of yeast P. pastoris, the mechanisms of mitophagy for this yeast is still unknown. At this stage of the project we obtained the recombinant strains of methylotrophic yeast P. pastoris, which carry the hybrid gene omp85 P. pastoris fused with GFP gene (coded the green fluorescent protein). These recombinant strains were obtained on the base of wild type strain of P. pastoris and on the base of the mutant with the lack of protease activity P. pastoris SND1163. the experiments in which we sellected the best conditions of the effective degradation of F1B-subunite of mitochondrical ATPase. For this the cells of wild type and mutant strains of P. pastoris were grown under the conditions of mitophagy induction on the minimal YNB medium with 1,5% of glycerol during 5 days. Then the samples for immunoblot analysis with the antibodies for GFP were prepared. Analysing the results of Western-blot analysis we can investigate the time and level of mitophagy in methylotrophic yeast P. pastoris. Product Description popup.authors Дмитрук Олена Валеріївна Левків Катерина Олександрівна Сибірний Андрій Андрійович popup.nrat_date 2020-04-02 Close