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Information × Registration Number 0215U000544, 0113U003656 , R & D reports Title The investigation of expresion of marker proteins epithelial stem cells and progenitor cells in oral squamous cell carcinoma. popup.stage_title Head Sydorenko Myhail V., Registration Date 27-02-2015 Organization Department of Biotechnical Problems of Diagnostics, Institute of Problems of Cryobiology and Cryomedicine of NAS of Ukraine popup.description2 Abstract. Research report 68 p., 4 tab., 14 fig., 52 literature sources. The purpose of the work. Examine the expression of marker proteins of epithelial stem and progenitor cells of the epithelium of the oral mucosa: CD44, ALDH1, SK5/14 P63 and assess the biological role of cells expressing these marker proteins; in squamous cell carcinoma of the oral cavity at all stages of the process flow of the tumor to treatment and after treatment. The object of study: the first group of normal mucosa biopsy (10 observations, men, age 32-68 years who did not smoke, material Bureau of Forensic Medicine); -biopsy second group of tumor and mucosa at a distance from the existing tumor treatment taken simultaneously (observation 13 men, age 43-79 years old, patients with squamous cell carcinoma of the oral cavity: 13 tumor biopsies mucosal biopsies and 13 at a distance from the tumor); third group - biopsy of the primary tumor to treatment and surgical material tumor metastasis, recurrence after treatment of the same patients (observations 13, males, age 40-70 years, 13 biopsies of the primary tumor and 13 tumors after treatment). Methods: histological, immunohistochemical. Changes in the oral mucosa and tumor were performed according to the criteria of international histological classification (UICC). Expression of marker proteins are estimated by immunohistochemical techniques monoclonal (Mouse Monoclonal anti CD44 HCAM (clone 156-3C11); anti-ALDH1A1, Product Number HPA002123, (SIGMA-Aldrich); Mouse Monoclonal Anti-Human CD44, Phagocytic Clycoprotein-1, clone DF1485, P63 antigen, clone 4A4, two cytokeratin antigen SK5/14 clone LL002 imaging system Mouse/Rabbit PolyVue (TM) HRP/DAB Detection System", (Diagnostic BioSystems, USA). The immunohistochemical reaction was evaluated by immunohistochemical labeling index of cells in Percentage (% MI) was determined and the labeled cells Histotopography MI (%) in the cell layers of the epithelium and mucosal tumors, as well as depending on the histopathological changes in the mucosa, clinical stage of the disease and on the TNM system level of tumor differentiation. at a distance from the mucous formed squamous cell carcinoma determined expression of marker proteins CD44, ALDH1, SK5/14, P63 in unusual for normal epithelial cells of the central and surface suprabasal layers, whose share increased depending on the stage histopathological progression; in the microscopic intraepithelial cancer, on the brink of invasion (the so-called invasive front) in squamous cell carcinoma of the 1st degree and a primary tumor in the same mucosa, indicating the involvement of cells expressing the marker proteins of epithelial stem and progenitor cells in the formation of the transformed phenotype, invasion and progression. In primary tumors of squamous cell carcinoma of the oral cavity after the base held a combined radiotherapy and chemotherapy and against various tumor response to treatment, and regardless of the number of courses of the therapy, clinical stage and tumor grade shown preservation of subpopulations of tumor cells with the phenotype of cancer stem cells CD44+ and ALDH1+ phenotype and epithelial stem cells SK5/14+, P63+, indicating that the resistance of these cells to modern schemes therapy of squamous cell carcinoma, leading to further tumor progression. Tags: oral squamous cell carcinoma, epithelial stem cells, cancer stem cells, marker proteins. Product Description popup.authors Вдовиченко Наталя Олександрівна Кірєєва Світлана Сергіївна Лебедєва Тамара Миколаївна Першко Наталя Юріївна Сидоренко Михайло Васильвич Юрченко Наталя Петрівна popup.nrat_date 2020-04-02 Close