Registration Number
0215U001001,
0114U002736
, R & D reports
Title
Molecular systems of winter wheat's genetical and epigenetical polymorphism detection introduction to receive of highproductive specialized varieties
popup.stage_title
Head
Grodzinsky Dmytro Mykhailovych,
Registration Date
12-01-2015
Organization
Institute of cell biology and genetic engineering NAS of Ukraine
popup.description2
Heterogeneity of a variety of winter soft wheat was discovered by the key economical features and the speed of seed germination and a ranking was made by their productivity and ecoligical flexibility. It was found that there exists a static connection between a species and an epigenetic polymorphis, ecoligical flexibility is connected to "epiginetical distance" between the profiles of DNA methylation, and the difference in profiles of methylation whit additional radiation influence depends on it's starting polymorphysm.Due to introduction of molecular systems of genetical and epigenetical polymorphism researchfor 100 of varieties of wheat : a screening was held to discover valuable aleles of genes; the custom DNA was sigularized and cleaned; the methods of uni and multiplex polymerase chain reactions where developed to discover target genes; the level of distribution of aleles of low and medium activity of polyphenoloxydative enzymes was determined and the validation of molecular systems was held. There was created a protocol for dectection of valuable aleles of genes. There were discoverd varieties of wheat with rye-wheat translocations 1AL.1RS and 1BL.1RS, recessive alele of gene Tamyb10, gene of seprtiosis resistance Stb4 which is hooked with a polymorphic locust Xgwm111. The variety of wax-wheat and a number of varieties that are the carriers of an atypical functional alele.
Product Description
popup.authors
Берестяна А.М.
Голубенко А.В.
Кравець О.П.
Кучук М.В.
Моргун Б.М.
Нітовська І.О.
Соколова Д.О.
Степаненко А.І.
Степаненко О.В.
Шнуренко О.Р.
popup.nrat_date
2020-04-02
