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Information × Registration Number 0215U001374, 0112U000099 , R & D reports Title Investigationof the epigenetic mechanismsof induced resistance to the toxc action of heayy metals at different stages of ontogenesis popup.stage_title Head Bozhkov A., Registration Date 16-02-2015 Organization Kharkov National University named after V.N. Karazin popup.description2 Object of study - epigenetic patterns, age-dependent characteristic epihenotypes. Experiments were performed on male rats 3 and 20 months of age using different regimens of copper sulfate and determining the lethal dose. Effect of fungidol determined in experiments on 3-month animals - rats Wistar male in compliance with bioethics. All animals determined changes in body weight, rectal temperature and time swimming with the load. Also used culture microalgae Dunaliella viridis Teod., which were cultured in a liquid medium Artari a modification, the initial concentration of 1.3 million cells / ml) at a constant temperature of 26-28 °C and the illumination (4 klk). Purpose - to develop epigenetic mechanisms induced resistance to toxic effects of heavy metals on different stages of ontogeny within the concept of epigenetic mechanisms of aging and concept epigenotype which dependent age. Methods - biochemical, determine the activity of glutathione peroxidase, glutathione-S-transferase, lipid hydroperoxides, determination of total lipids and ?-carotene in cells of algae, content of nucleic acids and protein, carbonyl protein, free proline, copper, determination of intracellular pattern of copper ions, allocation copper-bearing proteins, getting two sublines - CuS sensitive and resistant to copper ions CuR. These results confirm the role of metabolic memory formation hormesis effect and choose different adaptation strategies in young and old animals. Adaptation of microalgae Dunaliella viridis to lethal concentrations for this type of copper ions accompanied by the formation of specific epigenotype, which was characterized by accelerated as chronological and replicative senescence, which depended on the intensity transplants. It is shown that for CuR-subline D. viridis have a higher rate of chronological aging compared to CuS-subline. Fungidol stimulated the activity of glutathione peroxidase in mitochondria and microsomal fraction of liver cells fungidol a dose of 0.5 ml / kg had expressed antitoxic effect. In the event that the introduction of copper sulfate animals injected fungidol, the content and functional activity of red blood cells even after the introduction of copper sulfate did not differ from control option. It turned out that fungidol particularly effectively increased the antioxidant protective system at mitochondria. Antioxidant and antitoxic fungidol form a common adaptive metabolic pattern and appear as interrelated properties Product Description popup.authors А.Наглов В.Длубовська В.Малєєв В.Сидоров М.Асадова М.Гірич Н.Гернет Н.Кургузова С.Огієнко С.Шаповалов Т. Криворучко Ю.Кравченко popup.nrat_date 2020-04-02 Close