Search academic texts

Registration Number

0215U003049, 0114U000058 , R & D reports

Title

Investigation of efficacy of photodynamic therapy in treatment of infected radiation lesions of the skin.

Head

Simonova-Pushkar L.I.,

Registration Date

16-01-2015

Organization

Grigor'ev Institut for Medical Radiology

Description

Research object. Unified radiation injuries of the skin on the experimental model of local X-radiation in rats. Research objective. To increase effectiveness of treatment of infected radiological injuries of the skin through elaboration of a new method with use of photo-dynamic therapy. Research methods and equipment. General clinical, microbiological instrumental, statistical. X-ray unit and biological microscopes. Theoretical and practical results. It has been proved that microbial community of the intact skin of rats is distinct in heterogenicity of species composition. In case of X-radiation of skin area, it is characterized by increase of microbial contamination, appearance of additional potentially pathogenic and pathogenic microorganisms. The model of infected radiation ulcer of skin of rats with cultures S. aureus and P. аeruginosа and the scheme of effective antibacterial photo-dynamic therapy, which is based on selection of optimal parameters of action for photosensitizer of triethylene blue and source of photon heterochromatic photodiode emission in experiments in vitro, have been elaborated. It has been shown that photo-dynamic therapy which is carried out according to elaborated scheme aids in 100% elimination of gram-positive (S. aureus) as well as gram-negative (P. аeruginosа) microorganisms from the radiation ulcer surface. Novelty. Application of elaborated scheme of photo-dynamic therapy for elimination of pathogenic microflora from the surface of skin radiation injury was substantiated for the first time in experiments in vitro and in vivo on cultures of pathogenic microorganisms (S. aureus, P. аeruginosа). Implementation effectiveness: By means of the experiment it has been established that elaborated scheme of photo-dynamic therapy has significant antibacterial influence on potentially pathogenic and pathogenic microorganisms.Area of application - radiobiology.

Product Description

popup.authors

Білогурова Л.В.
Гертман В.З.
Коробов А.М.
Куров О.М.
Ляховська Н.В.
Маматкулова Т.М.
Мамуладзе М.Н.
Павліченко Ю.В.
Парадєєва З.С.
Сімонова-Пушкар Л.І.
Савельєва О.О.
Скляр Н.І.
Смєловська Г.В.
Трофименко Т.І.
Усик Г.В.