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Registration Number

0215U003379, 0110U005961 , R & D reports

Title

Immunoadjuvant properties of colloidal metal nanoparticles for oral immunization.

Head

Komisarenko Serhiy, Доктор біологічних наук

Registration Date

28-01-2015

Organization

A.V.Palladin Institute of Biochemistry of National Academy of Sciences

Description

. The development of modern approaches to oral immunization needs of new adjuvant carriers that have local mucosal immune-stimulating effect. These carriers may be nanoparticles of different nature that allow unhindered overcome mucous barriers and deliver antigen cells for the immune system. In order to find the most efficient carriers of antigen for per os immunization it was compared the characteristics of nanoparticles with different chemical structure and size (colloidal gold - Gold 1 and Gold 2, calcium phosphate - CAP and polylactide-co-glykolid PLGA 1 and 2). All the studied particles showed no significant cytotoxic impact to the studied cells in vitro, with the exception of CAP and Gold nanoparticles. The most pronounced phagocytosis by macrophage-like cell J774 was observed for PLGA nanoparticles 1 and 2 with the immobilized antigen. In BALB / c mice treated with oral antigen immobilized on PLGA 1 and 2 carriers, the concentration of specific IgA antibodies in the blood significantly increased after the first immunization, compared with controls. The results suggest promising research PLGA nanoparticles PLGA 1 and 2 as adjuvant for immunization per os.

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Галкін О.В.
Кабернюк А.А.
Колибо Д.В.
Короткевич Н.В.
Криніна О.І.
Лабинцев А.Ю
Манойлов К.Ю.
Олійник О.С.
Паливода К.О.
Редчук Т.А.
Романюк С.І.
Сіромолот А.А.
Чудіна Т.О.