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Information × Registration Number 0215U003402, 0110U005965 , R & D reports Title Immunoliposomes for the targeted delivery of bioactive compounds to tumor cells. popup.stage_title Head Komisarenko Serhiy, Доктор біологічних наук Registration Date 29-01-2015 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 Increased expression of Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is observed in a number of tumor types. Thus, this molecule could be an attractive target for antibody-based drug delivery into tumor cells. In this study, we generated single-chain variable fragment antibodies (scFv) to HB-EGF and used them for the construction of immunoliposomes. These immunoliposomes were evaluated for specific binding, internalization, and cytotoxicity for HB-EGF-expressing Vero cells. By means of flow cytometry and fluorescence microscopy it was demonstrated that HB-EGF-targeted immunoliposomes in contrast to nontargeted liposomes have been efficiently bound and internalized by Vero cells. It was shown that doxorubicin loaded immunoliposomes were cytotoxic for the HB-EGF-expressing cells in vitro. These results indicate that HB-EGF-targeted immunoliposomes provide efficient targeted delivery of anticancer drugs into HB-EGF-expressing cells. Product Description popup.authors Галкін О.В. Кабернюк А.А. Колибо Д.В. Короткевич Н.В. Криніна О.І. Лабинцев А.Ю Манойлов К.Ю. Олійник О.С. Паливода К.О. Редчук Т.А. Романюк С.І. Сіромолот А.А. Чудіна Т.О. popup.nrat_date 2020-04-02 Close