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Registration Number

0215U004231, R & D reports

Title

Molecular biological characteristics of phytopathogenic Agrobacterium, Erwinia, Ralstonia bacteria, their plasmids and bacteriophages resulting in killing activity

Head

Ivanytsia Volodymyr,

Registration Date

17-02-2015

Organization

Odesa I.I.Mechnikov national university.Pervomaisk institute.

Description

According to the technical task, molecular biological, biophysical and molecular genetic properties of bacteria Erwinia, Ralstonia and Agrobacterium, their plasmids, macromolecular bacteriocins and bacteriophages were investigated. For the first time it was shown that bacteriocines of Erwinia carotovora can be characterized by the wide diversity of the partickles of phage origin. Methods of antagonistic compound purification and methods of bacteria cultivation were optimized and developed. For the first time for Agrobacterium inhibition the strain-antagonist Alcaligenes faecalis was applied. For the first time, in the result of four cycles of inactivation-cultivation the stock of bacteriophage ZF40 rt2/4 containing temperature resistant phage partickles was obtained. For the first time the unique genetic stability of bacteriophage Erwinia carotovora ZF40 was revealed. Collections of phytopathogenic bacteria and their bacteriophages were created.

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