Registration Number
0215U006006,
0114U001594
, R & D reports
Title
The development and trial exploitation of a portative analyzer based on amperometric enzyme biosensors for quality control of beverages in winery. Development of biosensor methods of arginine analysis in wine by using arginine-hydrolyzing recombinant enzymes.
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Head
Sibirny Andrey,
Registration Date
21-01-2015
Organization
Institute of Cell Biology NAS of Ukraine
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Object of study: enzymes arginase I, argininedeiminase (ADI) and cells of recombinant yeasts - over-producers of arginase. Purpose of the research: to develop a laboratory prototype of amperometric biosensors on arginine based on recombinant enzymes (bacterial argininedeiminase and human liver arginase I) and the cells Hansenula polymorpha - over-producers of arginase; to test created biosensors on real samples of wines. As a result of the work performed in 2014: an arginine (L-Arg) selective enzymes (arginase and argininedeiminase) were obtained from the cells of recombinant yeast strains - over-producers of target enzymes and characterized. The laboratory prototypes of amperometric biosensors on L-Arg based on argininedeiminase, arginase and recombinant yeast cells with increased content of arginase were developed. An enzymatic method based on argininedeiminase on L-Arg with spectrophotometric mode of detection was developed and processed. The methods of L-Arg analysis were investigated on real samples of wines in comparison with referent chemical method. A strong correlation was shown to exist between the values obtained by the new and previously created methods. The developed methods based on arginine-hydrolizing enzymes was shown to possess the high validity during express-analyses of L-Arg levels in wines. According to the results of research 4 articles and 4 abstracts were published.
Product Description
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Іваш М.Ф.
Борецький Ю.Р.
Гайда Г.З.
Гончар. М.В.
Синенька М.М.
Стасюк Н.Є.
Фаюра Л.Р.
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2020-04-02
