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Registration Number

0215U008363, 0111U000977 , R & D reports

Title

Develop the scientific bases of creation of effective symbioses and associations of nitrogen-fixings bacteria with plants on condition of use adaptive potential of microorganisms, capable to endophytic growth.

Head

Nadkernychna Olena Volodymyrivna, Доктор біологічних наук

Registration Date

25-12-2015

Organization

Institute of agricultural microbiology and agro-industrial production of National academy of agrarian sciences of Ukraine

Description

Object of research - symbiotic and associative nitrogen-fixing bacteria, spring and winter wheat, spring triticale, soybean, common bean, Galega officinalis. The work purpose - to receive by methods of analytical selection the nitrogen-fixing bacteria capable to get into tissue of plants, to form effective symbioses and associations with plants and to promote activization of process of fixing of molecular nitrogen in their root zone, and also to investigate conditions of formation of effective symbioses and associations of legume and cereal crops with endophytic diazotrophs. Methods of researches: microbiological, molecular-genetic, serological, spectrometry, vegetative and field experiments, statistical. New perspective strains of bacteria which actively fix molecular nitrogen in symbiosis and in association with plants are received and protected by patents of Ukraine and are more effective, than standard strains. It is shown that active diazotroph A. brasilensе 102 at an introduction in a root zone of a spring wheat is capable to get into internal tissue of plants, forming effective endophytic association with plants. The method of estimation of nitrogen fixation potential of grain-crops is protected by the patent of Ukraine. Using it is possible to select sorts with hyperactivity of nitrogen fixation in a root zone of crop. The biopreparations Rizobofit and Rizogumin on the basis of a new highly effective strain of R. phaseoli FB1 for increase of productivity of common bean (for 11,0-36,4%) are offered. It is established that strains of nodule bacteria of soybean with different growth rate belong to different genetic groups and form two ITS-types. It is established that the greatest saprophyte competence characterizes intensive growing strains of KV11 serogroup of rhizobia. It is shown that for increase in productivity of soybean use of two active strains with the different growth rate of B. japonicum 46 and B. japonicum KV11 can be perspective. Methodical recommendations "Scientific bases of creation of artificial symbioses of diazotroph with cereal and legume crops " are developed. Scope of application - the microbiological industry, agriculture.

Product Description

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Копилов Є.П.
Крутило Д.В.
Надкернична О.В.
Павленко А.А.
Ушакова М.А.