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Information × Registration Number 0215U008525, 0111U003811 , R & D reports Title 3. The development and use of molecular technigues for identification of harmful organisms, transgenic constructs and genotypes of potato varieties to certification and study of genetic variability in the process of recovery. popup.stage_title Head Oliynyk T.M., Registration Date 28-12-2015 Organization Institute for potato Research popup.description2 Modified method of DNA isolation and conducted Boonham N. modification techniques for efficient isolation of RNA from the field leaves of potato plants. Were tested three methods of DNA isolation from bacteria R. solanacearum. Approved the methods of allocation of plasmid DNA agrobacterial strains. Fit, primers for diagnostic X, Y- and M viruses in potato plants probirkovyh potatoes. Exhaust conditions of the polymerase chain reaction with reverse transcription and shows the possibility of multiplexed reaction. Were tested test-system based on two-stage RT-PCR for the diagnosis of viral diseases VVBK and potato plants. Optimization of conditions and the selection of the reaction mixture concentrations of reagents and methods RAPD- ISSR-PCR to detect molecular genetic polymorphisms recovered lines. The possibility of holding ISSR-PCR using three ISSR primers combined in a single reaction. Conditions spent diagnosis of bacterial brown rot of potato R. solanacearum. A RAPD-analysis of nine strains of R. solanacearum, BOX-PCR and implemented clustering 10 strains of R. solanacearum. Optimized conditions of PCR analysis for the detection of genetic structures containing nptII gene neomitsynfosfotransferazy 35S promoter and VMKK. Product Description popup.authors Грицай Роман Васильович Слободян Сергій Олександрович popup.nrat_date 2020-04-02 Close