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Registration Number

0215U008527, 0111U003798 , R & D reports

Title

Biotechnological aspects of the creation and improvement of a new generation of potato varieties using DNA technology

Head

Oliynyk T.M.,

Registration Date

28-12-2015

Organization

Institute for potato Research

Description

Variation-improved way to create original fabric of potato breeding material using the method of cell selection. In the selective medium obtained regenerants population - 978 lines of various biotech varieties. In terms of population in vivo regenerants formed by the complex of commercially valuable indicators. 9 Highlight lines that are submitted for further testing. The analytical data evaluation process and hybrid breeding lines for biotechnology content: dry matter, starch, crude protein, carotenoids; assessment of resistance to darkening pulp raw and cooked state; drought index. Analysis hybrid selection process showed that the main components of the biochemical indicators of quality vary widely: -16,4-25,5% dry matter, crude protein - 1,7 -2,7% vitamin C -12,7-15,6 mg / 100g, carotenoids - 0,01-0,37 mg / 100g. Materials to develop schemes involving hybridization selection process. Developed provided direct induction meristem morphogenesis of different varieties of potatoes using the method kiltury apical meristem in combination with chemotherapy. Developed and published guidelines "Improvement of potato varieties apical meristem culture method in combination with chemotherapy." Developed a test system based on PCR for diagnosis and tuber viroids veretenopodibnosti X-, Y-, S-, L-, M- viruses and modern molecular genetic methods (RAPD- and ISSR-PCR) determine variability recovery following lines potato varieties biotech methods. The influence of external environmental factors on gene transcription palmitoleyiltransferazy experimental strains of R. solanacearum by RT-PCR. Fit, primers and conditions for PCR identification of gene-lipid A 3-hidroksymirystynoyiltransferazy analyzed sequenced sequence lipid A deatsylaz R. solanacearum.Vyyavleno that activation of lipid A 3-O-deatsylazy involved some component or combination contained in part of potato plants, and cleavage of 3-hidroksymirystatu important in the pathogenesis.

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Гуторчук Світлана Леонідівна
Конишева Ірина Миколаївна
Місяць Тетяна Костянтинівна
Сідакова Оксана Владиславівна
Слободян Сергій Олександрович