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Information × Registration Number 0215U008533, 0115U005687 , R & D reports Title Role of MRPS18-2 protein in regulation of cell cycle and cancerogenesis popup.stage_title Head Kashuba Olena Vitaliivna, Доктор біологічних наук Registration Date 28-12-2015 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology popup.description2 During the project S18-2 gene was cloned into the expression vector GFP-. GFP-fusion protein was expressed in embryonic fibroblasts mouse gene knockout on retinoblastoma (RH) resulting transfektsiy using Lipofectamine. Transfected cells were selected with growth in an environment with a marker G418. It was selected cell lines that expressed high levels of the protein GFP-S18-2 (RH-18) as shown by Western blot analysis and immunofluorescence microscopy in comparison to the original cell line. Also we have been induced GST-fusion proteins S18-2 and RB and RB mutant proteins and pure GST-protein in bacterial cultures (BL21 PLys) using IPTG. Purification was carried out by aid agencies carrier Sephacryl, coated with glutathione. The resulting fusion proteins were eluted using reduced glutathione buffer (without DTT). The quality and quantity of proteins on a carrier and eluted proteins was determined by Western blot analysis and Coomassie blue Product Description popup.authors Бучинська Любов Георгіївна Гордієнко Інна Михайлівна Кашуба Олена Віталіївна Ковалевська Лариса Миколаївна Чехун Василь Федорович Юрченко Наталія Петрівна popup.nrat_date 2020-04-02 Close