Registration Number
0215U008557,
0115U001343
, R & D reports
Title
Molecular-radiation Biotechnology for pharmacological efficacy increasing of medicinal plants in the culture and nature
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Head
Grodzinsky Dmytro Michailovich,
Registration Date
29-12-2015
Organization
Institute of cell biology and genetic engineering NAS of Ukraine
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Report on SRP: 25 pages., 6 figures, 5 tables, 22 references. The object of investigation - medicinal plants Matricaria chamomilla, Silybum marianum, Digitalis purpurea, Hypericum perforatum, Bidens tripartite, Salvia officinalis. The aim of research - developing radiation biotechnology to increase producing and isomeric variation secondary metabolites of medicinal plants in vitro for raise their pharmaceutical value. The methods of investigation - radiobiology, spectrophotometric, biochemical, physiological, statistical. The doses of seeds' acute irradiation were chosen and experimentally verified for medicinal plants: Matricaria chamomilla, Silybum marianum, Digitalis purpurea. It was shown that such doses led to increasing biomass and secondary metabolites synthesis. Literature research about growing medicinal plants methods, secondary metabolites (flavonoides) extracting and definition was also carried out. It was shown that presowing seeds' acute exposure led to increasing dry flower mass (under 5-50 Gy) and stimulating plant growth of Matricaria chamomilla (under 5 and 10 Gy). It was established that acute Matricaria chamomilla seeds' exposure under 5, 10, 20 and 35 Gy led to increased general yield of flavonoids extract per plant and per unit area of crop. Obtained data demonstrated the existence of positive correlation between irradiated seeds' germination and growth stimulation. Nevertheless, there was inverse relationship of flower amount per plant and average flower mass with seeds' germination. Silybum marianum seeds' radiation exposure under 10, 20, 35 and 50 Gy accelerated the plant passage into generative phase, but did not led to accelerated aging related to control. According to obtained data the thesis for International conference were prepared and accepted for publication.
Product Description
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Берестяна Анастасія Миколаївна
Жук Владислав Вікторович
Літвінов Сергій Вячеславович
Нестеренко Олена Геннадіївна
Пчеловська Світлана Анатоліївна
Салівон Анастасія Георгіївна
Соколова Дарина Олександрівна
Тонкаль Людмила Володимирівна
Шиліна Юлія Володимирівна
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2020-04-02
