Registration Number
0216U000016,
0113U000284
, R & D reports
Title
To investigate the antigenic properties of tumor stem cells of the brain malignant gliomas and to develop methods for specific immunoglobulins for immunodiagnostical and immunotherapeutical using.
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Head
Lisyaniy Nicolay,
Registration Date
14-01-2016
Organization
State institution ''Institute of Neurosurgery n.acad. AP. Romodanov NAMS of Ukraine''
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The object of research: tumors of the central nervous system, peripheral blood, blood serum. The goal: to get specific antibodies against tumor stem cells malignant gliomas of the brain for those immunodiagnostic immunotherapy use. Research methods: immunological methods, immunefluorescence, immunohistochemistry, cultural methods, experimental methods, cytofluorometry, microscopic methods. Results: As a result, poly-specific antisera and antibodies to mousses' brain embryonic neural cells (MBENC) and antisera are against stem tumor cells (STC). These antisera and antibodies against MBENC are made cytotoxic effect on different types of glioma and hampered proliferation in cell culture. In comparing monoclonal antibodies to CD133, antibodies to MBENC and received antisera detected those antisera are less bind to tumor cells, indicating the specificity of these antisera to stem cells. The method of concentration of STC by adhesion to lectin - fitohemahlyutynyni is created. Determined that content of CD133+ cells in gliomas increase with increasing the degree of malignancy. Investigation of immune status showed that high content of STP in the inhibition of tumor immune processes. Methods of obtaining antisera and allocation of specific antibodies can be used to further immunodiagnosis and immunotherapy, as they hampered the growth of tumor cells in culture. Areas of application implementation: to further scientific research and in clinical practice (neyroonkoloh, oncology, neurosurgery).
Product Description
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Єгорова Діана Михайлівна
Бєльська Людмила Миколаївна
Волкова Кристина В'ячиславівна
Главацький Олександр Якович
Гнєдкова Ірина Олександрівна
Ключнікова Антоніна Іванівна
Лісяний Микола Іванович
Лісяний Олександр Миколайович
Мазніченко Оксана Леонідівна
Малишева Тетяна Андріївна
Розуменко Володимир Давидович
Семенова Віра Михайлівна
Солодовченко Анастасія Генадівна
Стайно Лариса Петрівна
Станецька Діана Миколаївна
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2020-04-02
