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Information × Registration Number 0216U000026, 0114U006179 , R & D reports Title Identify changes in the level of expression of transforming growth factor beta in cultured glioma cells under conditions of antiproliferative influence of fetal rat neurogenic cells supernatant popup.stage_title Head Semenova Vira M., Registration Date 14-01-2016 Organization State institution ''Institute of Neurosurgery n.acad. AP. Romodanov NAMS of Ukraine'' popup.description2 Research report: 35 pages, 3 tables, 7 figures, 30 sources. Object of research - cell cultures of rat brain gliomas 101.8 and C6. Purpose - to study the relationship of the antiproliferative effects of the supernatant of neurogenic cells of fetal brain influence with TGF-beta - signaling in tumor cell culture of experimental rat brain glioma. Methods: the cultivation of tumor cells, immunocytochemical analysis of cell markers, morphometric analysis, enzyme immunoassay, statistical methods. It was found that the supernatants of rat's progenitor neurogenic cells (RPNS) derived from fetal brain of both early (E8-11), and later (E12-16) gestational age, showed cytotoxic effects on short-term 101.8 and C6 glioma cell cultures, the extent of which was dose-dependent and increases with increasing duration of incubation of cultures with the RPNS. RPNS (E12-16) showed a more pronounced cytotoxic effect on cultured C6 and 101.8 glioma cells, compared with the RPNS (E8-11). The cytotoxic index (CI) of RPNS (E12-16) towards C6 and 101.8 glioma cells significantly exceeded the CI towards a cell suspension of the normal rat brain. After exposure to the RPNS (E8-11) to the C6 and 101.8 glioma primary cultures the signs of dose-dependent cytotoxicity were showed: zone growth rarefaction, appearance of dystrophic and necrobiotic changes in tumor cells, mitotic index reducing, which intensified under the influence of the RPNS (E12-16). Under the influence of RPNS compared to the control observations, the average nucleocytoplasmic ratio in the tumor cells so as the proportion of cells with multiple nucleoli and cells immunopositive for Ki-67 reduced (2.7 times). The correlation between changes in the TGF- beta 1 expression level in cultured glioma cells with the proliferation marker Ki-67 expression level as a result of an anti-proliferative effect of the RPNS was found (r = 0,253). Scientific novelty. RPNS of fetal rat brain has a dose-dependent cytotoxic and antiproliferative effect on C6 and 101.8 glioma cultured cells, enhancing with increasing gestational age and duration of incubation of cell cultures with RPNS. A prerequisite for such an effect is likely to be the ability of neural progenitor cells to produce substances with anti-tumor effect. Practical significance. Established antiproliferative effect of the RPNS could be the basis for the theoretical study of complex pathogenetic therapy in patients with gliomas using drugs derived from fetal neurogenic cells. NEUROGENIC PROGENITOR CELLS, FETAL RAT BRAIN, SUPERNATANT, TUMOR CELL CULTURES, GLIOMA C6, GLIOMA 101.8, Ki-67, CYTOTOXIC INDEX, MITOTIC INDEX, PROLIFERATIVE INDEX, ANTIPROLIFERATIVE EFFECT. Product Description popup.authors Васлович В.В. Любич Л.Д. Малишева Т.А. Стайно Л.П. popup.nrat_date 2020-04-02 Close