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Registration Number

0216U001294, 0115U001992 , R & D reports

Title

Investigation of biocompatibility of allulose/hydkoxyapatite composites forbone tissue engineering

Head

Babenko Nataliya,

Registration Date

04-02-2016

Organization

Kharkov National University named after V.N. Karazin

Description

Isolated hepatocytes as well as extensor digitorum longus muscles of 3-month-old Wistar male rats were used. Spectrophotometric and radioisotope methods, as well as methods of cytology and statistic analysis were used in the study. The aim of the work was to compare the morphology of nano-hydroxyapatite/cellulose composites and their effects on viability and functional state of hepatocytes and extensor digitorum longus muscles. Generally, the morphology of the prepared hydroxyapatite/cellulose scaffolds for bone tissue engineering meets the requirements of materials which are implanted in the bone defect. All investigated composites, including scaffolds containing micro-hydroxyapatite/cellulose particles have demonstrated insignificant changes in cell viability and function, except nano-hydroxyapatite/cellulose scaffolds, where cell viability, as well as cell response to insulin action demonstrated significant drop in comparison to control cells. The determination of cytotoxicity, membrane integrity and metabolic effects of the scaffolds will be useful in the development of materials for regenerative medicine. Results obtained in the Department of Physiology of Ontogenesis of Kharkov Karazin National University on biocompatibility of nano-hydroxyapatite/cellulose composites for bone tissue engineering were presented and discussed on the joined with the Department of Organic Technology ( Kaunas University of Technology, Lithuania) video-conference and international conference Nanomaterials: Applications and Properties (Lviv, 2015)

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Гарькавенко В.
Стороженко Г.
Харченко В.
Шеверьова В.