Registration Number
0216U003067,
0114U000716
, R & D reports
Title
Preparation of stable tumor cell lines expressing phenotypic and molecular features of cancer stem cells (CSCs), to develop and monitor the effectiveness of new anticancer drugs: evaluation of mTOR/S6K-dependent signaling pathways
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Head
Filonenko Valeriy Viktorovitch, Доктор біологічних наук
Registration Date
12-01-2016
Organization
Institute of Molecular Biology and Genetics of NASU
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It was demonstrated that Ruk1 expression induced cell resistance inhibitor of mTORC1 rapamycin. This in turn may be related to the fact that the specific activation (phosphorylation) of key substrates of mTOR/C1 and mTOR/C2 - protein 4 EBP, S6K1 and Akt and their subcellular localization in cells with stable expression Ruk1 differs significantly from the original cells. A monoclonal antibody to components of mTOR/C1 and mTOR/C2 complexes proteins Rictor and Raptor have been generated and subcellular localization of signaling complexes in model cancer stem cells was analyzed
Product Description
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Кіямова Рамзія Галямівна
Маланчук Оксана Миколаївна
Овчаренко Галина Вікторівна
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2020-04-02
