Registration Number
0216U005325,
0115U003002
, R & D reports
Title
Biochemical and cellular links of the individual answer of organism on radioprotectors
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Head
Uzlenkova N.E.,
Registration Date
07-07-2016
Organization
Grigor'ev Institut for Medical Radiology
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Object of research: radiobiological experimental model for іn vіvo and іn vіtro estimations of efficacy of radioprotectors. Purpose: to define biochemical and cellular aspects of the individual variability of efficacy of radioprotectors. Methods: radiobiological, hematological, morphological, statistical. Equipment: x-ray installation RUM-17, universal dosimeter UNІDOS, auto hematology analyzer RT-7600, laminar case MN 120, incubator СО2 EU-160, centrifuge NF 800, microscope Leіca 6000B, chambers Leіca DFC 310 FX, microscope Leіca DM ІL LED, microscope Axіolab 94D 2601, object-micrometer. Theoretical and practical results: it was developed radiobiological model of acute radiation syndrome at total body x-irradiation at doses 4,0; 5,5; 7,0 and 8,2 Gy. It was determined the lethal dose response relationship using probit-analysis an LD50/30 - 6,85 Gy, LD16/30 - 4,46 Gy and LD84/30 - 7,97 Gy. The time of development and severity of the hematopoietic syndrome was established by counts of complete blood cells (leukocytes, lymphocytes, platelet and erythrocytes). Hematopoietic-relation parameters used as important in vivo endpoints of the hematopoietic acute radiation syndrome on the developed model. The maximum increase of apoptosis and hypoplasia with depression of granulocitopoiesis in bone marrow was determinant at the period from 1 to 3 days after irradiation. It was established that proliferation capacity of bone marrow's mesenchymal stem cells in culture is sensitive test system іn vіtro estimations of hematopoietic stromal compartment in bone marrow. Novelty: the study establishes a radiobiological model of acute radiation syndrome for іn vіvo and іn vіtro estimations of individual differences at total body x-irradiation. Efficiency: is not present. Use area: radiobiology.
Product Description
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Ігнатенко Ю.Г.
Васильєв Л.Л.
Дзюбенко Н.О.
Дригваль А.А.
Кривуля О.С.
Леонова І.О.
Магда І.Ю.
Маматкулова Т.М.
Масленнікова О.Л.
Ненюкова О.В.
Пасюга В.М.
Скоробогатова Н.Г.
Трофимов А.В.
Узлєнкова Н.Є.
Шелковая Л.П.
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2020-04-02
