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Information × Registration Number 0216U007578, 0116U006937 , R & D reports Title Molecular biological regulatory mechanisms of disturbance of fertilizing ability spermatozoa and the development of new immuno-biochemical diagnostic methods of fertility in men popup.stage_title Head Vorobets D.Z., Registration Date 22-12-2016 Organization Danylo Halytsky Lviv National Medical University popup.description2 It was established that the activity of arginase in the sperm of men with olihozoo-, astenozoo-, olihoastenozoo- and leykotsytospermiyeyu reduced by 2.1, 2.3, 2.4 and 3.3 times respectively. This indicates arhinaznoho suppression pathway of L-arginine, which is not significantly dependent on the type of disruption of spermatogenesis. However, reducing the activity of arginase is most pronounced in patients with leykotsytospermiyeyu because white blood cells stimulate the formation of reactive oxygen species, induction and development and oxidative stress in sperm nitrazyvnoho. There were also offset serum content gonadotropin and sex hormones in men with various forms pathospermia. In particular the content of follicle stimulating hormone with oligozoospermia caused by hypogonadism twice higher, and if leykotsytospermiyi 1.8 times on values ??in fertile men. However, the conditions astenozoospermiyi the figure below 2.2 times in relation to the values ??of normozoospermiyeyu men, but within the physiological norm. The content of testosterone below 1.6 times in men with impaired spermatogenesis oligozoospermia and the type is at the lower limit of the physiological norm. Found that infertile men with impaired spermatogenesis oligozoospermia the type arginase inhibition of sperm positively correlated with a decrease in their concentration in the ejaculate (r = 0,68). The features of NO-synthase pathway of L-arginine sperm in infertile men with various forms pathospermia. In men, fertility observed in violation of separation ( "uncoupling") NO-synthase sperm redistribution activities in the system of NO-synthase with their shift toward Ca2 + -independent inducible isoform, which indicates dismetabolic changes in the synthesis of NO, namely its hyperproduction. Among the various forms pathospermia in infertile men are most pronounced violations of NO-synthase pathway of L-arginine is observed in patients with leykotsytospermiyeyu. Our data indicate violations NO-homeostasis hyperproduction "harmful" NO involving activation of iNOS and nitrozyvnoho stress in the sperm of men with various forms patospermiy that is a violation of their functions, including the ability zaplidnyuvalnoyi. Changes in the performance spermatohram infertility correlated with increased levels of lipid peroxidation and reduced antioxidant enzyme activity hlutationovoyi system - glutathione peroxidase, glutathione reductase. It is assumed that chronic urogenital infections initially lead to activation of lipid peroxidation and inhibition of antioxidant enzymes sperm, but after this the development of pathological changes in the sperm of men and infertility. Defining the lipid peroxidation, antioxidant enzyme activity, activity arhinazno-NO-synthase systems sperm may be an additional test in the diagnosis of infertility in men. Product Description popup.authors Єфремова Уляна Петрівна Воробець Зіновій Дмитрович Онуфрович Олена Костянтинівна Фафула Роман Володимирович popup.nrat_date 2020-04-02 Close