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Information × Registration Number 0217U000130, 0113U007730 , R & D reports Title To investigate the efficiency of method fetal neuronal stem cells direct differentiation to the serotoninergic neurons and to determine their functional activity popup.stage_title Head Tsymbalyuk Vitaliy Ivanovich, Registration Date 19-01-2017 Organization Academician A.Romodanov Institute of neurosurgery AMSc of Ukraine popup.description2 Today the molecular mechanisms of serotonergic neurons specialization from the stem and progenitor nerve cells and their regenerative potential are not fully understood. The aim of the study was to determine the most effective neuroinductors to support the survivality and functional activity of serotonergic neurons, their determined precursors and tested the regenerative activity of cell population, obtained by this way, at molecular and genes level. The research was carried out on the suspension and slices culture of nerve cells n. raphe zone using histochemistry, IFA and PLR methods. It has been established, that BDNF and NGF stimulated the serotonergic neurons amount increasing in the dissociated culture at 1,6 and 2 times compared with the control slices. Adding BDNF and retinoic acid leads to increasing total serotonin content in the culture material. The Nkx2.2 genes expression, witch is the marker of the mitosis-active serotonergic progenitors, supports by BDNF and increased in the presence of EGF and FGFb. The gene Pet1 expression, witch indicates the presence of serotonergic determined precursors supports by all using factors, except exogenous serotonin. The gene Tph1 expression, marker of serotonin synthesis start, was observed in the presence of FGFb, EGF and NGF. Our results showed, that co-culturing of injured organotypic slices zone n. raphe with suspension of in vitro expanded serotonin progenitors, prevented the retrograde degeneration, promoted the morphological regeneration, increased the serotonin level and neuronal survivality. Moreover, it stimulated all serotonin regulated genes expression. So, our data shows that neuroinductors, listed here, plays their roles at the different phases of serotoninogenesis and can be using for it's regulation. Serotonergic cell population, obtained by this way, can effectively stimulated zone n. raphe regeneration after injury. Product Description popup.authors Бараненко Б.О. Васильєва І.Г. Галанта О.С. Дмитренко А.Б. Макарова Т.А. Олексенко Н.П. Цюбко.О.І. Чопик Н.Г. Шуба І.М. popup.nrat_date 2020-04-02 Close