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Information × Registration Number 0217U000138, 0113U007732 , R & D reports Title To investigate molecular and cell pathological compensatory processes of ischemic stroke for optimization of neurosurgery and therapeutic treatment popup.stage_title Head Tsymbalyuk Vitaliy Ivanovich, Registration Date 20-01-2017 Organization Academician A.Romodanov Institute of neurosurgery AMSc of Ukraine popup.description2 Research object is a venous blood of patients, suffered for ischemic stroke and healthy control. The aim of this study was to investigate the association between genetic and metabolic features of patients with ischemic stroke and risk of development and disease flowing. Methods: polymerase chain reaction followed digestion with restriction enzymes, immunocytochemistry, spectrophotometry. 78 patients with ischemic stroke, 12 patients with hemorragic stroke and 55 healthy controls were enrolled in this study. The presence of polymorphisms G894T, T-786C и 4a/4b (NOS3 gene); С677Т, A66G, 2756G та A1298С (folate cycle genes), С-148Т, G20210A, G1691A (plasma hemostasis genes); М235Т, I/D та Т174М (renin-angiotensin-aldosteron system genes) was detected in all groups. It was determined that polymorphic variants С-148Т (FGB gene), 4в4а, G894T ( NOS3 gene) and С677Т (MTHFR gene) are associated with early-onset of ischemic stroke in 18-65 years old Ukrainian Caucasian population. II genotype of I/D polymorphism (ACE gene) is a risk factor for ischemic stroke. Presence of 677T allele is associates with highest cholesterol and triglicerides level in blood plasma of ischemic stroke patients and healthy controls. Carriers of 677TT genotype had a highest level of CD105+ cells in venous blood the 677CT carriers and significant high difference of CD105+ cells level with 677CC carriers. Product Description popup.authors Васильєва І.Г. Галанта О.С. Дмитренко А.Б. Костюк М.Р. Макарова Т.А. Олексенко Н.П. Сніцар Н.Д. Цюбко О.І. Чопик Н.Г. Шуба І.М. popup.nrat_date 2020-04-02 Close