Registration Number
0217U000149,
0112U000259
, R & D reports
Title
To create the new strains of nitrogen-fixing bacteria with increased resistance to stress factors and to leam of physiological and biochemical characteristics of their interaction with plants
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Head
Kots Sergii Yaroslavovych,
Registration Date
23-01-2017
Organization
Institute of Plant Physiology and Genetics NASU
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As a result of the study it was shown that the pure cultures of nodule bacteria S.meliloti AC08 and AC88 had resistance to mineral nitrogen (KNO3) in concentrations of 100-200 mM. Transposon mutant T17 had resistance to 100 mM. As a result of alfalfa seed inoculation the root nodules were formed by above mentioned reisolates under introduction of both optimal and high (stress factors) doses of mineral nitrogen. These nodules had higher rate of nitrogen fixation as compared to the nodules formed by rhizobial strain 425a. It was noted that rhizobacteria RS1, A20, RP13 and RP5 generally stimulated the process of nodulation and increased the rate of nitrogen fixation during formation of the symbiosis between soybean and nodule bacteria B.japonicum 634b in greenhouse experiment. In this case the impact of isolates RP13 and A20 was more expressive. The changes in contents of both polysaccharides and proteins were observed while nodule bacteria were influenced by environmental stress factors and defense substances . It was shown that the application of physiologically active substances, in particular lectins, to treat the seeds or nodule bacteria (suspension for inoculation) provided soybean seed yield enhancement under insufficient water supply up to 10 and 20 per cent respectively.
Product Description
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Береговенко Світлана Кирилівна
Воробей Надія Анатоліївна
Грищук Олена Олександрівна
Жемойда Алла Володимирівна
Левішко Алла Сергіївна
Маліченко Світлана Марківна
Маменко Павло Миколайович
Мельник Вікторія Миколаївна
Мельникова Наталя Миколаївна
Михалків Людмила Миронівна
Омельчук Світлана Василівна
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2020-04-02
