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Information × Registration Number 0217U000688, 0114U001061 , R & D reports Title Develop an algorithm for histological examination to determine prognosis in colon adenocarcinoma with the coupling morphological, histochemical and immunohistochemical features popup.stage_title Head Grabovoi Alexandr Nikolaevich, Registration Date 21-02-2017 Organization Ukrainian Research Institute of Oncology and Radiology popup.description2 The object of study, adenocarcinoma of the rectum.Objective: To increase the reliability of individual prognosis of CRC species complexes objective by defining prognostic factors and develop their algorithm histologyconsidering ztsiplennya morphological, histochemical and immunohistochemical characteristics.Methods and apparatus: histological, histochemical, immunohistochemical, morphometric, densiometrychni, statistics; Laboratory pH meter, weight torsion.It was shown that DNA content in the nuclei of cells AKTK, due to the significant variability can not act as an independent criterion for determining tumor differentiation and individual prognosis. In the ranks of cell adenomas ranked by the amount of DNA in the nuclei there is a high degree of connection between the amount of DNA and a total nucleolar organizer (r = 0,92), and malignant tumors in this relationship is broken. It is shown that the variability of proliferative activity in a variety of AKTK G, and in benign tumors (2,17-98%, r = 0,58), does not allow a sufficient degree of confidence using MI-67 Key for determining the malignant potential of a single tumor. AKTK for correlation amount of DNA in the nuclei of cells and MI Ki-67 (r = from +1 to -1). The presence or absence of expression of Bcl-2 and p53 in AKTK generally not significantly different in tumors of different G. presence of Bcl-2 expression in tumor cells results in a significant (? = 0,416, p <0,05), than p53 (? = 0,313, p <0,05), an increase of DNA in cell nuclei AKTK. Heterogeneity of cellular AKTK the content of DNA in the nuclei of cells decreases with increasing G, which manifests itself increase the relative proportion of cells with nuclei that are most relevant to functionally active (p <0,01).Based on the equation Cox proportional to the intensity resulting model determining the probability of survival of good quality for patients with AKTK G2 and G3, which have increased the average content of DNA in the nuclei of tumor cells.The algorithm histological examination of this type of tumor. The latter provides after establishment of histological type and tumor differentiation conventional methods, determination of DNA in the nuclei of tumor cells, the number and volume of nucleolar organizers labeling index Ki-67 expression of Bcl-2 and p53 followed by calculation of G and the probability of survival to 60 months and the tumor can be attributed to favorable or not adverse histological type. Product Description popup.authors Антонюк Сергій Анатолійович Грабовий Олександр Миколайович Колеснік Олена Олександрівна Тарасова Тетяна Олександрівна popup.nrat_date 2020-04-02 Close