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Information × Registration Number 0217U001176, 0115U005201 , R & D reports Title Obtaining of cell lnes of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators popup.stage_title Head Galkin Anatoliy Pavlovich, Registration Date 26-01-2017 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 The wheat (TriticumaestivumL.) plant-regenerantsvariety Zimoyarkawere obtained in vitro conditions on the nutrient media MS with bioregulatorsof microbiological origin: Avercom, Avercom nova-2, Violar, Phytovit used at concentrations 20-100 ?l/l incomplexwith 1-2 mg/l BAP and 0,5-1 mg/l IAA.In vitroconditionsthe decrease of degree of infestation of wheatplant-regenerantsby parasitic cerealnematodeH.avenaeaccording tophenological parameters - up to 20-37% was found.The improvement of morphometricparameters ofwheatplant-regenerantsgrown ingreenhouse conditions on the natural invasive soil: the high of stems-on 113,6-137,5%, the length of the flagleaf and colossus - on 118-124%, the number of grains per colossus - on 40,6-56,3%, the weightof colossus- on 27,0-54,5% was observed.The difference in the percentage of hybridized moleculesmRNA and si/miRNAfrom cells of control and experimental plants grown both on a nutrient media withbioregulatorson the artificialinvasive background -up to 15-39% andin greenhouse conditions on the natural invasive soil - up to 33-56% was revealed. Inthewheat germ cell-free systemof protein synthesis theincreaseofsilencingactivityofsi/miRNAisolatedfromcells of experimental wheat plant-regenerants - upto 29-64% on the template of mRNA from plant-regenerants, and up to 20-37%on the template of mRNA fromnematode larvae was shown. Product Description popup.authors Іутинська Г.О. Білявська Н.О. Галкін А.П. Циганкова В.А. Шиша О.М. popup.nrat_date 2020-04-02 Close