Registration Number
0217U001176,
0115U005201
, R & D reports
Title
Obtaining of cell lnes of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators
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Head
Galkin Anatoliy Pavlovich,
Registration Date
26-01-2017
Organization
Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine
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The wheat (TriticumaestivumL.) plant-regenerantsvariety Zimoyarkawere obtained in vitro conditions on the nutrient media MS with bioregulatorsof microbiological origin: Avercom, Avercom nova-2, Violar, Phytovit used at concentrations 20-100 ?l/l incomplexwith 1-2 mg/l BAP and 0,5-1 mg/l IAA.In vitroconditionsthe decrease of degree of infestation of wheatplant-regenerantsby parasitic cerealnematodeH.avenaeaccording tophenological parameters - up to 20-37% was found.The improvement of morphometricparameters ofwheatplant-regenerantsgrown ingreenhouse conditions on the natural invasive soil: the high of stems-on 113,6-137,5%, the length of the flagleaf and colossus - on 118-124%, the number of grains per colossus - on 40,6-56,3%, the weightof colossus- on 27,0-54,5% was observed.The difference in the percentage of hybridized moleculesmRNA and si/miRNAfrom cells of control and experimental plants grown both on a nutrient media withbioregulatorson the artificialinvasive background -up to 15-39% andin greenhouse conditions on the natural invasive soil - up to 33-56% was revealed. Inthewheat germ cell-free systemof protein synthesis theincreaseofsilencingactivityofsi/miRNAisolatedfromcells of experimental wheat plant-regenerants - upto 29-64% on the template of mRNA from plant-regenerants, and up to 20-37%on the template of mRNA fromnematode larvae was shown.
Product Description
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Іутинська Г.О.
Білявська Н.О.
Галкін А.П.
Циганкова В.А.
Шиша О.М.
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2020-04-02
