Search academic texts

Information × Registration Number 0217U001500, 0115U000486 , R & D reports Title Elaboration of the theory of experimental regulation of duration ontogenesis popup.stage_title Head Bozhkov A., Registration Date 21-02-2017 Organization Kharkov National University named after V.N. Karazin popup.description2 The object of study - the state of prooxidant-antioxidant system of rats under accelerated aging. Purpose - to study the aging process on an experimental model of bone marrow cells. Methods - the method of cultivation cell culture, biochemical, immunological. The intensity of proliferation in primary cell culture expressed in KM more so in old animals compared to the young. This indicates that the number of cells CM able to proliferate in older animals superior to young animals. After introducing exogenous copper ions revealed a dose-dependent response to CM cell culture which was different to the culture of cells obtained from young and old animals. Age-dependent nature of the reaction cell responses km copper sulfate maintained regardless of the state of the organism (intact or Cu-induced fibrosis). However, in older animals with liver fibrosis morphotypes pattern changed little compared with their intact animals - slightly increased content of band neutrophils, lymphocytes did not change the content of metamyelocytes, segmented neutrophils, myelocytes, eosinophils, basophils and monocytes. Young and old animals differed not only by the total number of identified morphotypes, but the ratio of these cells (allocation pattern). Preservation of band neutrophils in primary culture was very high. In young animals on the 96th hour of cultivation remained 60% neutrophils stab if they were obtained in young intact animals and 41% if - in animals with liver fibrosis. The number of cells CM able to proliferate in culture old animals, superior or inferior to young animals not on this indicator. The intensity and nature of the growth curve of growth of cell cultures KM young (3 months). Animal liver with fibrosis did not differ from that for intact animals; Cu-induced liver fibrosis influenced both the rate of growth (it was significantly reduced, compared to intact animals), and the character - it was unusual rhythmic character. Cell response KM culture in vitro, who stood in animals with fibrosis can be associated with different cell culture composition of young and old animals Product Description popup.authors А. Бондар В. Марченко М. Асадова М. Гуменна Н. Кургузова С. Огієнко Ю.Кузнецова popup.nrat_date 2020-04-02 Close