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Information × Registration Number 0217U001501, 0115U000485 , R & D reports Title Developing the concept of "dynamic functional networks" on vegetable and animal models popup.stage_title Head Kuznetsova U., Registration Date 21-02-2017 Organization Kharkov National University named after V.N. Karazin popup.description2 The objects of study were wheat seedlings in hydroponic culture; Culture of bone marrow cells of rats.The purpose of the work. Obtaining experimental data to develop a theory of the dynamics of functional systems based on concepts developed by us autoregulation on the example of the "root-microenvironment" and cell culture, further development of the theory of "epigenetic mechanisms of aging." - Research Methods - cytological, culture of bone marrow cells, physiological, biochemical, immunological, statistical methods of evaluation. Revealed that sodium fluoride in doses of 10 mm and high temperature (34 ° C) caused marked inhibition of root growth of seedlings of wheat from the 1st to the 3rd day of growth in hydroponic culture. Fever caused a more pronounced compared with sodium fluoride inhibit root growth. Found that the roots of wheat seedlings in the control group increased proline content of 16.54 mg proline / mg protein 24 per hour increase to 26.59 mg proline / mg protein 72 per hour growth. At the roots of seedling wheat grown in medium with 10 mM sodium fluoride on the 24th hour of growth, proline content was 2 times higher than in controls. In the second and third day of growth proline content in roots of seedlings dostvirno experimental group did not differ from controls. At the roots of seedlings that grew at an elevated temperature (34 ° C), the content of proline 24 per hour growth was not significantly different from the control, and the second and third day of growth increased by 72 and 98%, respectively, compared with control . Adding sodium fluoride on the third day led to growth inhibition of excretion of carbohydrates: their content is in control of the 48 th to the 72nd hour growth increased by 3 times, while growth in the medium with 10 mM sodium fluoride - only 1.7 times. However, the intensity increased excretion of protein and amino acids - did not differ from controls. Cultivation of wheat seedlings in a medium with 10 mM NaF and at a temperature of 34 ° C was accompanied by inhibition of the growth of seedlings. Inhibition of root growth NaF not accompanied by an increase of proline content in roots at elevated temperatures proline content in the roots of wheat seedlings increased in all experimental variants. Inhibition of root growth NaF and fever accompanied by increased excretion of protein, carbohydrates and amino acids. Pattern ratio of different components in the root ekzometabolitah was different for different variants of cultivation. The intensity of the growth of bone marrow cells in culture was different when they got in intact animals, animals with Cu-induced fibrosis, and fibrosis of the animals treated with CCM. Morphologically in the bone marrow, highlighted in rats can identify eight types of cells (lymphocytes, stab and segmented neutrophils, myelocytes, metamyelocytes, basophils, monocytes, eosinophils). Pattern morphotypes was different in the bone marrow intact animals, animals with liver fibrosis and fibrosis of the animals treated with CCM. Number of lymphocytes in the bone marrow of animals with Cu-induced liver fibrosis was significantly higher than in intact animals and animals with fibrosis who received CCM and the neutrophil count was lower in animals with fibrosis in animals with fibrosis who received CCM, compared with bone marrow cells intact animals. Neutrophils isolated from bone marrow fibrosis of the liver of animals treated with CCM, twice as fast as the system collapsed in vitro in primary culture than cells obtained in intact animals. The experimental data support the concept of autoregulation in the "root-microenvironment" Product Description popup.authors А. Бондар М. Асадова М. Ковальова С. Огієнко popup.nrat_date 2020-04-02 Close