Registration Number
0217U003342,
0112U003134
, R & D reports
Title
Study of functional activity and structural characteristics of tumor stem cells after cryopreservation and interaction with cells of fetal origin
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Head
Goltsev Anatoly Mykolayovuch,
Registration Date
30-03-2017
Organization
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine
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The objects of study were mouse C3H/He, CBA/H, C57BL and Balb/c lines; fetal liver cells (CFP) C57BL mice. Purpose of study was to estimate the effect of cryopreservation and introduction of products of fetal origin on the structural and functional characteristics of cancer stem cells (CSC) in the development of cancer pathology in different forms of its manifestation. Modern methods in the research were used: flow cytometric, imunomagnetic separation method, PCR, cultivation in vivo, cryopreservation. A comparative analysis of diiferent mode was conducted. Repopulation activity features and structural characteristics of present therein progenitor tumor cells was assessed. The ability of FLC which were cryopreserved by selected mode to correct of the T-cell immunity state was demonstrated. The dose-dependent nature of this kind of cryopreserved FLC activity was shown.The results are the basis for early diagnosis, understanding of the pathophysiology of cancer and decryption of therapeutic action mechanisms of fetal material. The results which reflect the response of tumor cells of different level of differentiation to cryoinfluence can be used to optimize the methods of cryoablation of malignancies.
Product Description
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Бабенко Наталя Миколаївна
Бондарович Микола Олександрович
Гаєвська Юлія Олександрівна
Гольцев Анатолій Миколайович
Дубрава Тетяна Георгіївна
Останков Максим Вадимович
Останкова Людмила Василівна
Порожан Євгенія Олександрівна
Челомбитько Ольга Васильївна
Ямпольська Катерина Євгеніївна
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2020-04-02
