Registration Number
0217U006290,
0116U000228
, R & D reports
Title
To study the biological properties of mesenchymal stem cells of mammals, to develop the technology for their production, cryopreservation and culture
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Head
Gerilovych Anton Pavlovych,
Registration Date
15-03-2017
Organization
Nationali Scientific Center "Institute of Experimental and Clinical Veterinary Medicine"
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The optimum technology for in vivo obtaining mesenchymal stem cells in bone marrow dog. It is established that due to anatomic structure, lack postmanipulyatsiynyh complications as well as the convenience of the procedure for the selection of the bone marrow in dogs is most suitable iliac bone. Use for fractionation of bone marrow cell suspension glucose-citrate solution with polyglukin is more efficient by eliminating the need to further launder cells from fluid gradient that can receive up to 86% of viable cells. Defined cellular composition of a preparation of mesenchymal stem cell bone marrow dogs. Most are erythroid cells (50%). Leukocyte composition varied. Unlike the peripheral blood in the bone marrow is dominated by immature young forms of neutrophils, and is up 4% progenitor cells of leukocytes, miyelotsyt and metamiyelotsyt
Product Description
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Келеберда Микола Іванович
Кузнецов Євген Петрович
Фісенко Світлана Анатоліївна
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2020-04-02