Registration Number
0218U000427,
0115U000055
, R & D reports
Title
Research of the correlation between neutrophil apoptosis and phagocytic mechanisms and deseases activity, clinical signs in patients with systemic deseaes of connactive tissue.
popup.stage_title
Head
Potomkina Galina,
Registration Date
30-01-2018
Organization
Danylo Halytskyy Lviv National Medical University
popup.description2
The object of the study - patients with systemic diseases of connective tissue (SDCT) and acute inflammatory processes of the abdominal cavity (acute pancreatitis and pancreatic necrosis). The purpose of the study was toResearch of the correlation between apoptosis and secondary necrosis of granulocytes and mononuclear cells (lymphocytes and monocytes) with the degree of activity and clinical manifestations of SDCT in order to improve the diagnosis and determine the degree of activity of the basic therapy. Methods of research - flow cytometry, microscopy, immunohistochemical, statistical methods of investigation research. Using Apolect technology, it has been established that granulocyte and mononuclear cell necrosis was significantly higher in chronic inflammation in SDCT patients, particularly in patients with systemic scleroderma and systemic lupus erythematosus. Instead, the apoptosis of granulocytes and mononuclear cells in the blood was the highest in patients with mixed SDCT. On the basis of the obtained data, the ROC curve was used to determine the sensitivity (69.5%) and specificity (88.8%) of Apolect technology and the number of necrotic and apoptotic leukocytes in a group of healthy individuals, which did not exceed 3.0%. Comprehensive immunological studies and determination of apoptotic and necrotic leukocytes will allow a more differentiated approach to the choice of basic therapy regimens for the treatment of SDCT. In order to prevent the formation of autoimmune complications in patients after acute inflammatory processes and prevent the increase of autoaggression in patients with SDCT, a promising method of using lectin-conjugated magnetic microparticles is recommended. Field of application: clinical immunology, rheumatology, surgery, cardiology.
Product Description
popup.authors
Білий Ростислав Олександрович
Бойко Я.Є.
Вовк Володимир Іванович
Гаєвська Віра Юріївна
Гаврилюк Анна Мирославівна
Горбаль Наталя Миронівна
Думич Тетяна Іванівна
Зубченко світлана Олександрівна
Климишин Дмитро Олександрович
Кріль Ірина Йосипівна
Ліщук-Якимович Христина Олександрівна
Личковська Наталя Едуардівна
Ломіковська Марта Павлівна
Потьомкіна Галина Олександрівна
Синенький Омелян Володимирович
Стефанишин Юрій Михайлович
Толстяк Ярослав Федорович
Фірман І.А.
Ханас О.І.
Чопяк Валентина Володимирівна
popup.nrat_date
2020-04-02
